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The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Human peripheral blood mononuclear cells were stimulated with CD3 (UCHT1), CD28 (CD28.2), and recombinant human IL-2 for 24 hours; and then stained with CD4 PerCP, LAP (TGF-β) PE and GARP (7B11) APC (top) or mouse IgG2b, κ APC isotype control (bottom). Data was analyzed by gating on CD4-positive cells.
Glycoprotein A Repetitions Predominant (GARP), also known as leucine rich repeat containing 32 (LRC32), is a 80 kD type I membrane glycoprotein with 20 leucine rich repeats in the extracellular portion of the protein. GARP was found on the surface of megakaryocytes, platelets, and activated Tregs (CD4+, CD25+, FoxP3+ cells) and serves as a receptor for latent TGF-β. Recent evidence suggests that GARP may play a role in controlling suppressor function of Tregs. A mutation in GARP has been reported in a large Samaritan kindred with Usher syndrome type 1, an autosomal recessive disease characterized by profound congenital sensorineural deafness, vestibular dysfunction, and progressive visual loss. In addition, it has been found that GARP mRNA is highly amplified in different tumors, which indicates that tumor cells may use GARP to express TGF-β or to capture TGF-β from their surroundings, resulting in local suppression of anti-tumor immune responses or the induction of Tregs.
Other Names:
Garpin, Glycoprotein A repetitions predominant
Structure:
80 kD transmembrane GARP glycoprotein with an extracellular region containing 20 leucine-rich repeats
Distribution:
Activated Tregs, megakaryocytes, platelets
Ligand Receptor:
LAP (TGF-β1)
Antigen References:
1. Ollendorff V, et al. 1994. Cell. Growth Differ. 5:213. 2. Stockis J, et al. 2009. Eur. J. Immunol. 39:3315. 3. Wang R, et al. 2009. P. Natl. Acad. Sci. USA 106:13439. 4. Tran DQ, et al. 2009. P. Natl. Acad. Sci. USA 106:13445.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human GARP (LRRC32)
Human platelets were stained with purified GARP (clone 7B11) (filled histogram) or mouse IgG2b, κ isotype control (open histogram), followed by anti-mouse IgG PE.
PE anti-human GARP (LRRC32)
Human peripheral blood mononuclear cells were stimulated with CD3 (UCHT1), CD28 (CD28.2), and recombinant human IL-2 for 24 hours; and then surface stained with CD4 FITC, GARP (7B11) PE (top) or mouse IgG2b, κ PE isotype control (middle), followed by intracellular staining with FOXP3 Alexa Fluor® 647; or surface stained with CD4 PerCP, GARP (clone 7B11) PE and LAP/TGF-β1 APC (bottom). The data was analyzed on CD4-positive cells.
APC anti-human GARP (LRRC32)
Human peripheral blood mononuclear cells were stimulated with CD3 (UCHT1), CD28 (CD28.2), and recombinant human IL-2 for 24 hours; and then stained with CD4 PerCP, LAP (TGF-β) PE and GARP (7B11) APC (top) or mouse IgG2b, κ APC isotype control (bottom). Data was analyzed by gating on CD4-positive cells.
