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The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application Notes:
The 1B4C3 monclonal antibody recognizes human erbB3/HER3 also know as oncogene erbB3, tyrosine kinase cell surface receptor HER3, and C-erbB3.
Human breast cancer cell line MCF-7 stained with 1B4C3 APC
erbB3/HER3 is a receptor tyrosine kinase and a member of the epidermal growth factor receptor family. erbB3/HER3 binds the heregulin ligands and contains 4 furin-like repeats, 2 cheY receiver domains, and a tyrosine kinase domain with a predicted molecular weight approximately 148 kD. Signaling through the erbB3/HER3 receptor induces proliferation and differentiation in some cell types, the receptor is widely expressed in many tissues including kidney, lung, brain, placenta, skin and stomach. erbB3/HER3 forms constitutively active heterodimers with erbB2/HER2 in a subset of human mammary tumors; expression of erbB3/HER3 reported be linked to favorable outcome in bladder and ovarian cancer. erbB3/HER3 has been shown to interact with a number of proteins including EGF receptor, erbB2/HER2, erbB4, Grb2, Grb7, SOS1, cdk5, SHC, FAK, PTK6, and others. The erbB3/HER3 receptor is extensively phosphorylated at residues T873 and S1123 (by cdk5); Y1199, Y1262, Y1289, and Y1328. The 1B4C3 antibody has been shown to be useful for flow cytometric detection of human erbB3/HER3.
Member of the epidermal growth factor receptor family. A receptor tyrosine kinase containing 4 furin-like repeats, 2 cheY receiver domains, and a tyrosine kinase domain. Predicted molecular weight approximately 148 kD
Distribution:
Widely expressed in many tissues including kidney, lung, brain, placenta, skin and stomach. Forms constitutively active heterodimers with erbB2/HER2 in a subset of human mammary tumors; expression of erbB3/HER3 reported be linked to favorable outcome in b
Function:
Induces extensive signaling cascade that can regulate growth and possibly differentiation
1. Carraway KL, et al. 1994. J. Biol. Chem. 269:14303. 2. Kraus MH, et al. 1989. Proc. Natl. Acad. Sci. USA 86:9193. 3. Chan SD, et al. 1995. J. Biol. Chem. 270:22608. 4. Tanner B, et al. 2006. J. Clin. Oncol. (Epub Aug. 8 2006)
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human erbB3/HER-3
Human breast cancer cell line MCF-7 stained with purified 1B4C3, followed by biotinylated anti-mouse IgG and Sav-PE
Biotin anti-human erbB3/HER-3
Human breast cancer cell line, MCF-7, stained with biotinylated 1B4C3, followed by Sav-PE
PE anti-human erbB3/HER-3
Human breast cancer cell line MCF-7 stained with 1B4C3 PE
APC anti-human erbB3/HER-3
Human breast cancer cell line MCF-7 stained with 1B4C3 APC
