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APC anti-human CD56 (NCAM) Antibody
APC anti-human CD56 (NCAM) Antibody
318309 25 tests $125.00     
318310 100 tests $265.00     
Product Details
Clone: HCD56
Isotype: Mouse IgG1, κ
Isotype Control:APC Mouse IgG1, κ Isotype Ctrl (FC)
Reactivity: Human
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Storage & Handling: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested
Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Enter Lot#:   
Application
References:

1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
Human peripheral blood lymphocytes stained
Human peripheral blood lymphocytes stained with HCD56 APC


Compare all formats



Description:

CD56 is a single transmembrane glycoprotein also known as NCAM (Neural Cell Adhesion Molecule), Leu-19, or NKH1. It is a member of the Ig superfamily. The 140 kD isoform is expressed on NK cells and NK-T cells. CD56 is also expressed in brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias also express CD56. CD56 plays a role in homophilic and heterophilic adhesion via binding to itself or heparin sulfate.
Other Names: Leu-19, NKH1
Structure: Ig superfamily, single transmembrane or GPI-anchored glycoprotein
Distribution: NK cells, T subset, neural tissue, some LGL and myeloid leukemias
Function: Adhesion
Ligand Receptor: Heparin sulfate
Antigen
References:

1. Lanier L, et al. 1991. J. Immunol. 146:4421.
2. Hemperly J, et al. 1990. J. Mol. Neurosci. 2:71.
3. Cremer H, et al. 1994. Nature 367:455.
GeneID: 4684
Latest Publications: View the latest CD56 articles on HighwirePress.com
UniProt: View information about CD56 on UniProt.org
Keywords: APC anti-human CD56 (NCAM), HCD56, APC, Leu-19, NKH1, Human, Immunology, Antibodies
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
  • Purified anti-human CD56 (NCAM)
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with purified HCD56,then detected with anti-mouse IgG FITC

  • FITC anti-human CD56 (NCAM)
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HCD56 FITC

  • PE anti-human CD56 (NCAM)
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HCD56 PE

  • PE/Cy5 anti-human CD56 (NCAM)
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HCD56 PE/Cy5

  • APC anti-human CD56 (NCAM)
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HCD56 APC

  • Alexa Fluor® 488 anti-human CD56 (NCAM)
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HCD56 Alexa Fluor® 488

  • Alexa Fluor® 647 anti-human CD56 (NCAM)
    Human NK-92 cells were stained

    Human NK-92 cells were stained with CD56 (clone HCD56) Alexa Fluor® 647. Cells were imaged with a Zeiss Axio Observer Z1 spinning disc confocal, average exposure time ~0.23 seconds. Credit: Dr. Jordan Orange and Dr. Emily Mace, University of Pennsylvania School of Medicine Children's Hospital of Philadelphia, Division of Immunology.

    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HCD56 Alexa Fluor® 647

  • Alexa Fluor® 700 anti-human CD56 (NCAM)
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with CD16 (3G8) PE and HCD56 Alexa Fluor® 700

  • PE/Cy7 anti-human CD56 (NCAM)
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HCD56 PE/Cy7

  • Biotin anti-human CD56 (NCAM)
    Human NK-92 cells were stained

    Human NK-92 cells were stained with CD56 (clone HCD56) Biotin, and then secondarily stained with Streptavidin-Alexa Fluor® 488. Cells were imaged with a Zeiss Axio Observer Z1 spinning disc confocal, average exposure time ~0.23 seconds. Credit: Dr. Jordan Orange and Dr. Emily Mace, University of Pennsylvania School of Medicine Children's Hospital of Philadelphia, Division of Immunology.

    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with biotinylated HCD56, followed by Sav-PE

  • PerCP/Cy5.5 anti-human CD56 (NCAM)
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HCD56 PerCP/Cy5.5

  • LEAF™ Purified anti-human CD56 (NCAM)
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with LEAF™ purified HCD56, followed by anti-mouse IgG FITC

  • Pacific Blue™ anti-human CD56 (NCAM)
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HCD56 Pacific Blue™

  • APC/Cy7 anti-human CD56 (NCAM)
    Human peripheral lymphocytes were stained

    Human peripheral lymphocytes were stained with CD16 FITC and CD56 (clone HCD56) APC/Cy7 (top) or mouse IgG1 APC/Cy7 isotype control (bottom).





  • Brilliant Violet 421™ anti-human CD56 (NCAM)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 FITC and CD56 (clone HCD56) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).





  • Brilliant Violet 570™ anti-human CD56 (NCAM)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 FITC and CD56 (clone HCD56) Brilliant Violet 570™ (top) or mouse IgG1, κ Brilliant Violet 570™ isotype control (bottom).





  • Brilliant Violet 605™ anti-human CD56 (NCAM)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 FITC and CD56 (clone HCD56) Brilliant Violet 605™.

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