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The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections.
Application References:
1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
Human peripheral blood lymphocytes stained with HI186 APC
CD52, also known as Cambridge pathology antigen 1 (CAMPATH-1), is a 25-29 kD glycoprotein containing a large N-linked carbohydrate moiety. The actual molecule of CD52 is only 8-9 kD. It is expressed in the male reproductive tract and on virtually all lymphocytes (T and B cells), as well as macrophages/monocytes, eosinophils, and red cells. CD52 is thought to play a role in carrying and orienting carbohydrates. CD52 is a potent target for complement-mediated lysis and antibody-mediated cellular cytotoxicity and has been used as a depletion target for chronic lymphocytic leukemia (CLL)/lymphoma and immunosuppression. The HI186 antibody is useful for flow cytometry and immunohistochemistry.
Other Names:
Cambridge pathology antigen 1 (CAMPATH-1), epididymal secretory protein 1
Male reproductive tract (epididymis, seminal vesicle), T cells, B cells, macrophage/monocyte, eosinophil, erythrocytes, malignant lymphocytes
Function:
Thought to play a role in carrying and orienting carbohydrates. Target for complement-mediated lysis and antibody-dependent cellular cytotoxicity, used as a depletion target for chronic lymphocytic leukemia/lymphoma therapy and immunosuppression.
Antigen References:
1. Leukocyte Typing VI. Kishimoto T, et al. (Eds.) Garland Publishing Inc. (1997) 2. Xia MQ, et al. 1991. Eur. J. Immunol. 21:1677. 3. Kirchhoff C, et al. 1993. Mol. Reprod. Dev. 34:8. 4. Xia MQ, et al. 1993. Biochem. J. 293:633.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD52
Human peripheral blood lymphocytes stained with purified HI186, followed by anti-mouse IgGs FITC
FITC anti-human CD52
Human peripheral blood lymphocytes stained with HI186 FITC
PE anti-human CD52
Human peripheral blood lymphocytes stained with HI186 PE
APC anti-human CD52
Human peripheral blood lymphocytes stained with HI186 APC
