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APC anti-human CD43 Antibody
APC anti-human CD43 Antibody
343205 25 tests $85.00 Qty:
    
343206 100 tests $210.00 Qty:
    

Product Details

 
Clone: CD43-10G7 (See other available formats)
Isotype: Mouse IgG1, κ
Isotype Control:APC Mouse IgG1, κ Isotype Ctrl
Reactivity: Human
Immunogen: MV4-11 cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.

Excitation
Laser:
Red Laser (633 nm)
COA:
Enter Lot#:   
Application
References:

  Publication Library
APC anti-human CD43 Antibody CD43-10G7 Image 1
Human peripheral blood lymphocytes stained with CD3 (UCHT1) PE and CD43-10G7 APC (top) or mIgG1,k APC isotype control (bottom)


Compare all formats

See APC spectral data



Cell Surface Immunofluorescence Staining Protocol




Description:

CD43, also known as Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, and W3/13, is a large sigle chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites. It has been reported that CD43 binds to CD54 and Siglec-1. CD43 plays dual roles in cell adhesion and anti-adhesion, as well as costimulation of T cell activation and survival, and induction of apoptosis of T cells and hematopoietic progenitors.

Other Names: Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein
Structure: Single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites, 95-130kD
Distribution: Most hematopoietic cells including peripheral T cells, NK cells, granulocytes, monocytes, thymocytes, platelets, plasma cells, activated B cells, and hemotopoietic progenitor cells.
Function: Play a dual roles in cell-cell adhesion and anti-adhesion, costimulation of cell activation and survival, induce apoptosis of T cells and hematopoietic progenitors
Ligand Receptor: CD54, Siglec-1
Antigen
References:

1. Neil Barclay A, et al. 1997. The Leucocyte Antigen Facts Book:Second Edition. Page 238
2. Killeen A, et al. 1987. EMBO J. 6(13):4029.
3. Thurman EC, et al. 1998. Intl. Immunol. 10:691.
4. Onami TM, et al. 2002. J. Immunol. 168:6022.
5. Tong J, et al. 2004. J. Exp. Med. 199:1277.
6. Jones AT, et al. 1994. J. Immunol. 153:3426.

GeneID: 6693
Latest Publications: View the latest CD43 articles on HighwirePress.com
UniProt: View information about CD43 on UniProt.org
Keywords: APC anti-human CD43, CD43-10G7, APC, Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, Human, Flow Cytometry, Immunology, Antibodies


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Human TruStain FcX™ (Fc Receptor Blocking Solution)FC, ICC, ICFC


Other Formats

DescriptionCloneApplications
PE anti-human CD43CD43-10G7FC
Purified anti-human CD43CD43-10G7FC, IP


Related Pathways

Pathway
Regulatory T Cells
TH1 Pathway (Cellular Immune Response)
TH2 Pathway (Humoral Immune Response)


Related Categories

Category
Immunology > Human Immunology > NK/NKT > CD43
Immunology > Human Immunology > Monocyte/Macrophage > CD43
Immunology > Human Immunology > CD and Related Molecules > CD43 (Leukosialin) > CD43-10G7
Immunology > Human Immunology > Adhesion Molecules > CD43 > CD43-10G7

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 1 Revision Date: 2012-11-30
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-human CD43
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with CD43-10G7 PE and CD3 APC (top) or mouse IgG1, PE κ isotype control and CD3 APC (bottom).





  • PE anti-human CD43
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with CD43-10G7 PE and CD3 APC (top) or mouse IgG1, κ PE isotype control and CD3 APC (bottom).





  • APC anti-human CD43
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with CD3 (UCHT1) PE and CD43-10G7 APC (top) or mIgG1,k APC isotype control (bottom)

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