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The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, Westerm blotting, and inhibition of platelet aggregation2. The LEAF™ Purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 303908).
Application References:
1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. 2. Takahashi R, et al. 1999. Blood 93:1951.
Human peripheral blood platelets stained with HIP1 APC
CD42b is a 145 kD glycoprotein known as gpIbα. It is composed in a heterodimer with a β chain (CD42c, gpIbβ) through a disulfide bond. CD42b is expressed on platelets and megakaryocytes. CD42b/c heterodimer forms a complex with CD42a and d and acts as the receptor for Von Willibrand factor and thrombin.
Other Names:
Glycocalicin, gpIbα
Structure:
Mucin, leucine-rich repeat family, 145 kD
Distribution:
Platelets, megakaryocytes
Function:
Complex with CD42a, c and d, platelet adhesion and activation
Ligand Receptor:
Von Willebrand factor, thrombin
Antigen References:
1. Clemetson K, et al. 1982. J. Clin. Invest. 70:304. 2. Fox J, et al. 1988. J. Biol. Chem. 263:4882. 3. Kuijpers R, et al. 1992 Blood 79:283.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
FITC anti-human CD42b
Human peripheral blood platelets stained with purified HIP1 FITC
LEAF™ Purified anti-human CD42b
Human platelets stained with
HIP1 PE
PE anti-human CD42b
Human platelets stained with
HIP1 PE
Purified anti-human CD42b
Human peripheral blood platelets stained with purified HIP1, followed by anti-mouse IgGs FITC
PerCP anti-human CD42b
Human peripheral blood platelets stained with HIP1 PerCP
APC anti-human CD42b
Human peripheral blood platelets stained with HIP1 APC
