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APC anti-human CD4 Antibody
APC anti-human CD4 Antibody
300514 100 tests $140.00 Qty:
    
300537 500 tests $525.00 Qty:
    

Product Details

 
Clone: RPA-T4 (See other available formats)
Isotype: Mouse IgG1, κ
Isotype Control:APC Mouse IgG1, κ Isotype Ctrl
Workshop
Number:
IV T114
Reactivity: Human, Cross-Reactivity: Chimpanzee
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Storage & Handling: The CD4 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.

Excitation
Laser:
Red Laser (633 nm)
COA:
Enter Lot#:   
Application
Notes:

The RPA-T4 antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding and inhibit syncytia formation. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections3,4,5, and blocking of T cell activation1,2. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 300516).

Application
References:

  Publication Library
APC anti-human CD4 Antibody RPA-T4 Image 1
Human peripheral blood lymphocytes stained with RPA-T4 PE


Compare all formats

See APC spectral data



Cell Surface Immunofluorescence Staining Protocol




Description:

CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules, and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16.

Other Names: T4
Structure: Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution: T cell subset, majority of thymocytes, monocytes/macrophages
Function: MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand Receptor: MHC class II molecules, HIV gp120, IL-16
Antigen
References:

1. Center D, et al. 1996. Immunol. Today 17:476.
2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.

GeneID: 920
Latest Publications: View the latest CD4 articles on HighwirePress.com
UniProt: View information about CD4 on UniProt.org
Keywords: APC anti-human CD4, RPA-T4, APC, T4, Human, Cross-Reactivity: Chimpanzee, Flow Cytometry, Immunology, Antibodies


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Product FAQs

 
I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
For a complete list of FAQs on all our products, visit our FAQ page.
 
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Other Formats

DescriptionCloneApplications
Alexa Fluor® 488 anti-human CD4RPA-T4FC, IF
Alexa Fluor® 594 anti-human CD4RPA-T4IF
Alexa Fluor® 647 anti-human CD4RPA-T4FC
Alexa Fluor® 700 anti-human CD4RPA-T4FC
APC/Cy7 anti-human CD4RPA-T4FC
Biotin anti-human CD4RPA-T4FC
Brilliant Violet 421™ anti-human CD4RPA-T4FC, IF
Brilliant Violet 510™ anti-human CD4RPA-T4FC
Brilliant Violet 570™ anti-human CD4RPA-T4FC
FITC anti-human CD4RPA-T4FC
LEAF™ Purified anti-human CD4RPA-T4Block, FC, IHC, CyTOF®
Pacific Blue™ anti-human CD4RPA-T4FC
PE anti-human CD4RPA-T4FC
PE/Cy5 anti-human CD4RPA-T4FC
PE/Cy7 anti-human CD4RPA-T4FC
PerCP anti-human CD4RPA-T4FC
PerCP/Cy5.5 anti-human CD4RPA-T4FC
Purified anti-human CD4RPA-T4FC, IHC, CyTOF®
Purified anti-human CD4 (MaxPar® Ready)RPA-T4FC, CyTOF®


Related Pathways

Pathway
Cancer Immunoediting
CD4 and CD8 T-Cell Lineage
Chemokine Receptor Biology
CTLA4 Signaling
Dendritic Cells
Gamma-Delta T cells
Hematopoiesis from Pluripotent Stem Cell
ICos-ICosL Pathway in T-Helper Cell
IL-2 Pathway
Immunologic Networks 2011
ITK and TCR Signaling
MDSC-induced Immune Suppression
PKC-θ Pathway
Regulatory T Cells
T Follicular Helper Cell Pathway
T-Cell Receptor Signaling
TH1 Pathway (Cellular Immune Response)
Th17 Biology
TH2 Pathway (Humoral Immune Response)
Tregs in Tumor Escape


Related Categories

Category
Immunology > Non-Human Primate Immunology > CD4
Immunology > Human Immunology > Th1/Th2 > CD4
Immunology > Human Immunology > T Regulatory Cells (TReg) > CD4 (T4)
Immunology > Human Immunology > Stem Cells > CD4 (T4)
Immunology > Human Immunology > Dendritic cells > CD4 > RPA-T4
Immunology > Human Immunology > CD and Related Molecules > CD4 (T4, L3T4) > RPA-T4
Immunology > Human Immunology > B Cells > CD4 > RPA-T4
Immunology > Human Immunology > Costimulatory Molecules > CD4 > RPA-T4
Immunology > Human Immunology > Th17 > CD4 > RPA-T4
Immunology > Human Immunology > Monocyte/Macrophage > CD4 > RPA-T4

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 1 Revision Date: 2012-11-30
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • APC anti-human CD4
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with RPA-T4 PE

  • Biotin anti-human CD4
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with biotinylated RPA-T4, followed by Sav-PE

  • FITC anti-human CD4
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with RPA-T4 FITC

  • LEAF™ Purified anti-human CD4
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with LEAF™ purified RPA-T4, followed by anti-mouse IgGs FITC

  • PE anti-human CD4
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with RPA-T4 PE

  • PE/Cy5 anti-human CD4
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with RPA-T4 PE/Cy5

  • PE/Cy7 anti-human CD4
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with RPA-T4 PE/Cy7

  • Purified anti-human CD4
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with purified RPA-T4, followed by anti-mouse IgGs FITC

  • APC/Cy7 anti-human CD4
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with RPA-T4 APC/Cy7

  • Alexa Fluor® 488 anti-human CD4
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with RPA-T4 Alexa Fluor® 488

    Human peripheral blood mononuclear cells

    Human peripheral blood mononuclear cells were fixed with 1% paraformaldehyde (PFA), and then stained with 5 µg/ml of CD4 (clone RPA-T4) Alexa Fluor® 488 for 30 minutes at room temperature. The image was captured by 40X objective.

    Human peripheral blood mononuclear cells

    Human peripheral blood mononuclear cells were fixed with 2% paraformaldehyde, then stained with 5 µg/ml anti-human CD8 (clone RPA-T8) Alexa Fluor® 594 (red) and 5 µg/ml anti-human CD4 (clone RPA-T4) Alexa Fluor® 488 (green) for 30 minutes at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 40X objective.

  • Alexa Fluor® 647 anti-human CD4
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with RPA-T4 Alexa Flour® 647

  • Pacific Blue™ anti-human CD4
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with RPA-T4 Pacific Blue™

  • Brilliant Violet 421™ anti-human CD4
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 FITC and CD4 (clone RPA-T4) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).





    Human peripheral mononuclear cells were

    Human peripheral mononuclear cells were fixed with 1% Paraformaldehyde (PFA), and then stained with 5 µg/ml of CD4 (clone RPA-T4) Brilliant Violet 421™ for 30 minutes at room temperature. The image was captured by 40X objective.

  • Alexa Fluor® 700 anti-human CD4
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with RPA-T4 Alexa Fluor® 700

  • PerCP anti-human CD4
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with RPA-T4 PerCP

  • PerCP/Cy5.5 anti-human CD4
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with RPA-T4 PerCP/Cy5.5

  • Brilliant Violet 570™ anti-human CD4
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 FITC and CD4 (clone RPA-T4) Brilliant Violet 570™ (top) or mouse IgG1, κ Brilliant Violet 570™ isotype control (bottom).





  • Purified anti-human CD4 (MaxPar® Ready)
    Human PBMCs stained with 154Sm-anti-CD45

    Human PBMCs stained with 154Sm-anti-CD45 (HI30) and 145Nd-anti-CD4 (RPA-T4).

  • Alexa Fluor® 594 anti-human CD4
    Human peripheral blood mononuclear cells

    Human peripheral blood mononuclear cells were fixed with 2% paraformaldehyde (PFA), then stained with 10 µg/ml of CD4 (clone RPA-T4) Alexa Fluor® 594 (cyan), 10 µg/ml CD8 (clone RPA-T8) Brilliant Violet 421™ (blue), and 10 µg/ml CD19 (clone HIB19) Alexa Fluor® 488 (green) for 30 minutes at room temperature. Nuclei were counterstained with DRAQ5 and are shown in red. The image was captured with a 40X objective.

  • Brilliant Violet 510™ anti-human CD4
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD4 (clone RPA-T4) Brilliant Violet 510™ (filled histogram) or mouse IgG1, κ Brilliant Violet 510™ isotype control (open histogram).

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