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The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
The 67A4 antibody recognizes human CD324 also known as E-cadherin, cadherin-1, and UVO. CD324, a member of the cadherin superfamily, is a calcium-dependent, transmembrane cell-cell adhesion glycoprotein composed of 4 extracellular cadherin repeats and a highly conserved cytoplasmic tail region with a predicted molecular weight of approximately 100 kD. CD324 is widely expressed in epithelial cells in the colon, uterus, liver, keratinocytes, brain, heart, muscle, kidney, and pancreas, as well as erythroid cells. CD324 functions as a cell adhesion molecule involved in development, bacterial pathogenesis, and tumor invasion. In bacterial pathogenesis, the ectodomain of CD324 mediates bacterial adhesion to mammalian cells, while the cytoplasmic domain is required for internalization. CD324 binds to the αEβ7 integrin to mediate cell adhesion and also interacts with a number of intracellular proteins including including erbin, ezrin, caspase-3, caspase 8, β-catenin, presenilin 1, casein kinase II , as well as other extracellular proteins including the EGF receptor. CD324 is phosphorylated on multiple residues (S857, S866, S870, S872), and can be proteolytically cleaved at reside D769 by caspase-3. The 67A4 antibody has been shown to be useful for flow cytometry.
Other Names:
E-Cadherin, cadherin-1, and UVO
Structure:
Member of the cadherin superfamily. Calcium-dependent, transmembrane cell-cell adhesion glycoprotein composed of 4 extracellular cadherin repeats and a highly conserved cytoplasmic tail region. Predicted molecular weight approximately 100 kD.
Distribution:
Widely expressed in epithelial cells in the colon, uterus, liver, keratinocytes, brain, heart, muscle, kidney, and pancreas, as well as erythroid cells
Function:
Cell adhesion molecule involved in development, bacterial pathogenesis, and tumor invasion. The ectodomain of CD324 mediates bacterial adhesion to mammalian cell, while the cytoplasmic domain is required for internalization.
Ligand Receptor:
αEβ7 integrin
Modification:
Phosphorylated on multiple residues (S857, S866, S870, S872), proteolytically cleaved at reside D769 by caspase-3.
Interaction:
Interacts with a variety of proteins including erbin, ezrin, caspase-3, caspase 8, EGF receptor, β-catenin, presenilin 1, casein kinase II, and others
Antigen References:
1. Overduin M, et al. 1995. Science 267:386. 2. Boggon TJ, et al. 2002. Science 296:1303. 3. Berx G, et al. 1995. EMBO J. 14:6107. 4. Perl AK, et al. 1998. Nature 392:190.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD324 (E-Cadherin)
Human colon carcinoma cell line (HT29) stained with purified CD324 (clone 67A4) (filled histogram), or purified mouse IgG1, κ (open histogram) followed by anti-mouse IgG FITC.
BT474 breast cancer cells were stained with anti-human CD324 (clone 67A4) using 1:100 dilution, followed by DyLight™ 649 anti-mouse Ig secondary antibody (red) plus DAPI staining for nuclei (blue). Cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100, blocked with 10% serum, and incubated O/N at 4°C. Images were taken under 20x (filter set: EX 647/10x, Dichroic 665LP, EM 700/70x) at exposure 4s. Data provided by John Nolan and Er Liu, La Jolla Bioengineering Institute.
FITC anti-human CD324 (E-Cadherin)
Human colon carcinoma cell line (HT29) stained with CD324 (clone 67A4) FITC (filled histogram), or mouse IgG1, κ FITC (open histogram).
PE anti-human CD324 (E-Cadherin)
Human colon carcinoma cell line (HT29) stained with CD324 (clone 67A4) PE (filled histogram), or mouse IgG1, κ PE (open histogram).
APC anti-human CD324 (E-Cadherin)
Human colon carcinoma cell line (HT29) stained with CD324 (clone 67A4) APC (filled histogram), or mouse IgG1, κ APC (open histogram).
Alexa Fluor® 488 anti-human CD324 (E-Cadherin)
Human colon carcinoma cell line (HT29) stained with CD324 (clone 67A4) Alexa Fluor® 488 (filled histogram), or mouse IgG1, κ Alexa Fluor® 488 (open histogram).
Alexa Fluor® 647 anti-human CD324 (E-Cadherin)
Human colon carcinoma cell line (HT29) stained with CD324 (clone 67A4) Alexa Fluor® 647 (filled histogram), or mouse IgG1, κ Alexa Fluor® 647 (open histogram).
PerCP/Cy5.5 anti-human CD324 (E-Cadherin)
Human colon carcinoma cell line (HT29) stained with CD324 (clone 67A4) PerCP/Cy5.5 (red histogram), or mouse IgG1, κ PerCP/Cy5.5 (green histogram).
