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The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application References:
1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. 2. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
PHA-stimulated (3 day) human peripheral blood lymphocytes were stained were with CD25 (clone BC96) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).
CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.
Other Names:
Low affinity IL-2R, IL-2R α chain, Tac, p55
Structure:
Type I transmembrane glycoprotein, 55 kD
Distribution:
Activated T and B cells, monocytes/macrophages, Treg
Function:
Associates with IL-2 receptor β (CD122) and γ chains (CD132) to form high affinity IL-2R complex
Ligand Receptor:
IL-2
Antigen References:
1. Taniguchi T, et al. 1993. Cell 73:5. 2. Waldmann T. 1991. J. Biol. Chem. 266:2681.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-human CD25
PHA-stimulated (3 day) human peripheral blood lymphocytes were stained were with CD25 (clone BC96) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).
FITC anti-human CD25
PHA-stimulated (3 day) human peripheral blood lymphocytes were stained with CD25 (clone BC96) FITC (filled histogram) or mouse IgG1, κ FITC isotype control (open histogram).
PE anti-human CD25
PHA-stimulated (3 day) human peripheral blood lymphocytes were stained with CD25 (clone BC96) PE (solid line) or mouse IgG1, κ PE isotype control (dotted line).
PE/Cy5 anti-human CD25
PHA-stimulated (3 day) human peripheral blood lymphocytes were stained with CD25 (clone BC96) PE/Cy5 (filled histogram) or mouse IgG1, κ PE/Cy5 isotype control (open histogram).
Purified anti-human CD25
PHA-stimulated (3 day) human peripheral blood lymphocytes were stained with purified CD25 (clone BC96) (solid line) or purified mouse IgG1, κ isotype control (dotted line).
APC/Cy7 anti-human CD25
PHA-stimulated (3 day) human peripheral blood lymphocytes were stained with CD25 (clone BC96) APC/Cy7 (blue histogram) or mouse IgG1, κ APC/Cy7 isotype control (black histogram).
PE/Cy7 anti-human CD25
PHA-stimulated (3 day) human peripheral blood lymphocytes were stained with CD25 (clone BC96) PE/Cy7 (solid line) or mouse IgG1, κ PE/Cy7 isotype control (dotted line).
Alexa Fluor® 488 anti-human CD25
PHA-stimulated (3 day) human peripheral blood lymphocytes were stained with CD25 (clone BC96) Alexa Flour® 488 (filled histogram) or mouse IgG1, κ Alexa Flour® 488 isotype control (open histogram).
Alexa Fluor® 647 anti-human CD25
PHA-stimulated (3 day) human peripheral blood lymphocytes were stained with CD25 (clone BC96) Alexa Flour® 647 (filled histogram) or mouse IgG1, κ Alexa Flour® 647 isotype control (open histogram).
Pacific Blue™ anti-human CD25
PHA-stimulated (3 day) human peripheral blood lymphocytes were stained with CD25 (clone BC96) Pacific Blue™ (filled histogram) or mouse IgG1, κ Pacific Blue™ isotype control (open histogram).
Alexa Fluor® 700 anti-human CD25
PHA-stimulated (3 day) human peripheral blood lymphocytes were stained with CD25 (clone BC96) Alexa Flour® 700 (filled histogram) or mouse IgG1, κ Alexa Flour® 700 isotype control (open histogram).
Biotin anti-human CD25
PHA-stimulated (3 day) human peripheral blood lymphocytes were stained with biotinylated CD25 (clone BC96) (filled histogram) or biotinylated mouse IgG1, κ isotype control (open histogram).
PerCP/Cy5.5 anti-human CD25
PHA-stimulated (3 day) human peripheral blood lymphocytes were stained with CD25 (clone BC96) PerCP/Cy5.5 (filled histogram) or mouse IgG1, κ PerCP/Cy5.5 isotype control (open histogram).
Brilliant Violet 421™ anti-human CD25
Human peripheral blood lymphocytes were stained with CD4 FITC and CD25 (clone BC96) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
Brilliant Violet 605™ anti-human CD25
Human peripheral blood lymphocytes were stained with CD4 FITC and CD25 (clone BC96) Brilliant Violet 605™.
