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The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application References:
1. Sugden B and Metzenberg S. 1983. J. Virol. 46:800-807.
Human peripheral blood lymphocytes stained with CD19 PE and EBVCS-5 APC
CD23 is a 45 kD protein, also known as Leu-20, FcεRII, IgE Fc receptor, BLAST-2, B6, and low affinity IgE receptor. It is a member of the Ig family, expressed on most mature B cells, B cells in follicular mantle (but not in proliferating germinal center cells, follicular dendritic cells, monocytes, eosinophils, Langerhans cells, and a subset of T cells (10-15% of tonsillar T cells). CD23 responds to high levels of IgE by downregulating IgE secretion. In human monocytes, CD23 triggering results in release of pro-inflammatory cytokines including TNF-α, IL-1, IL-6, and GM-CSF. CD23 can be proteolytically cleaved to generate soluble CD23 fragments of various molecular weights. In chronic lymphocytic leukemia, levels of soluble CD23 in the serum can be used as a prognostic marker to identify patients at high risk for disease progression. Alternate splicing of exon 2 can also generate two cell-surface isoforms of CD23 differing by 6 amino acids in their cytoplasmic region. The D3.6 antibody against human CD23 has been shown to be useful for flow cytometry.
Ig family member, type II transmembrane glycoprotein, 45 kD.
Distribution:
B cells, follicular dendritic cells, monocytes, eosinophils, a subset of T cells, Langerhans cells
Function:
Regulation of IgE synthesis. In human monocytes, CD23 triggering results in release of pro-inflammatory cytokines including TNF-a, IL-1, IL-6, and GM-CSF
Ligand Receptor:
IgE, CD19/CD21/CD81
Antigen References:
1. Ludin C, et al. 1987. EMBO J. 6:109. 2. Delespesse G, et al. 1992. Immunol. Rev. 125:77. 3. Flores-Romo L, et al. 1993. Science 261:1038. 4. Armant M, et al. 1994. J. Exp. Med. 180:1005.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD23
Human peripheral blood lymphocytes stained with CD19 (HIB19) APC and EBVCS-5 PE
FITC anti-human CD23
Human peripheral blood lymphocytes stained with CD19 APC and EBVCS-5 FITC
PE anti-human CD23
Human peripheral blood lymphocytes stained with CD19 (HIB19) APC and EBVCS-5 PE
APC anti-human CD23
Human peripheral blood lymphocytes stained with CD19 PE and EBVCS-5 APC
PE/Cy7 anti-human CD23
Human peripheral blood lymphocytes stained with CD19 (HIB19) APC and EBVCS-5 PE/Cy7
PerCP/Cy5.5 anti-human CD23
Human peripheral blood lymphocytes stained with CD19 FITC and EBVCS-5 PerCP/Cy5.5
APC/Cy7 anti-human CD23
Human peripheral blood lymphocytes were stained with CD19 PE and CD23 (clone EBVCS-5) APC/Cy7 (top) or mouse IgG1 APC/Cy7 isotype control (bottom).
