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The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application Notes:
The E11/19 monoclonal antibody binds to phosphoserine 349 corresponding to 22 c-terminal amino acid residues of human CCR5 and is useful for flow cytometric analysis, immunoblotting, and ELISA assay of CCR5 expression. Additional reported applications (for the relevant formats) include: immunofluorescence microscopy1, and ELISA detection1 when paired with T21/8 as the capture antibody (Cat. No. 321401/321402).
Application References:
1. Pollok-Kopp B, et al. 2003. J. Biol. Chem. 278:2190. (IF ELISA) 2. Fox JM, et al. 2011. 117:1851. PubMed
PMA (100 ng/ml)-stimulated (5 minutes) human peripheral blood lymphocytes surface stained with CD3 FITC and intracellularly stained with CD195 (clone E11/19) APC (top) or mouse IgG1, κ APC isotype control (bottom).
CD195, also known as CCR5, is a 45 kD G protein-coupled seven transmembrane CC-chemokine receptor. It binds to MIP-1α, MIP-1β, and RANTES and is expressed on a subset of T cells and monocytes. CD195 mediates an intracellular signal thought to induce cell differentiation and proliferation. CCR5 has also been shown to act as a co-receptor for R5 HIV-1 cell entry; modification of CCR5 by sulfation contributes to the efficiency of HIV-1 entry.
Other Names:
CD195, CCR5, C-C chemokine receptor type 5, HIV-1 fusion co-receptor
Structure:
G-coupled receptor family 1, membrane protein, 45 kD
Distribution:
Subset of T cells, monocytes
Function:
Binds C-C chemokines and transduces an intracellular signal thought to result in proliferation and differentiation. Acts as a co-receptor with CD4 for HIV-1.
Ligand Receptor:
MIP-1a, MIP-1ß, RANTES
Antigen References:
1. Samson M, et al. 1996. Biochemistry 35:3362. 2. Raport CJ, et al. 1996. J. Biol. Chem. 271:17161. 3. Combadiere C, et al. 1996. J. Leukoc. Biol. 60:147. 4. Deng H, et al. 1996. Nature 381:661.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
PE anti-human CD195 (CCR5) Phospho (Ser349)
PMA (100ng/ml)-stimulated for 5 min (green histogram) and non-stimulated (red opened histogram) human peripheral blood lymphocytes intracellular stained with E11/19 PE
APC anti-human CD195 (CCR5) Phospho (Ser349)
PMA (100 ng/ml)-stimulated (5 minutes) human peripheral blood lymphocytes surface stained with CD3 FITC and intracellularly stained with CD195 (clone E11/19) APC (top) or mouse IgG1, κ APC isotype control (bottom).
