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The CD16 antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Storage & Handling:
The CD16 antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application Notes:
The 3G8 antibody blocks neutrophil phagocytosis and stimulates NK cell proliferation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections6, immunoprecipitation3, stimulation of NK cell proliferation4, blocking of phagocytosis5, and blocking of immunoglobulin binding to FcγRIII7,8. The LEAF™ Purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 302014).
Application References:
1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. 2. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. 3. Edberg J, et al. 1997. J. Immunol. 159:3849. (IP) 4. Hoshino S, et al. 1991. Blood 78:3232. (Stim) 5. Tamm A, et al. 1996. Immunol. 157:1576. (Block) 6. Da Silva DM, et al. 2001. Int. Immunol. 13:633. (IHC) 7. Holl V, et al. 2004. J. Immunol. 173:6274. (Block) 8. Hober D, et al. 2002. J. Gen. Virol. 83:2169. (Block) 9. Brainard DM, et al. 2009. J. Virol. 83:7305. PubMed 10. Smed-Sörensen A, et al. 2008. Blood 111:5037. (Block) PubMed 11. Timmerman KL, et al. 2008. J. Leukoc. Biol. 84:1271. (FC) PubMed 12. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) 13. Rout N, et al. 2010. PLoS One 5:e9787. (FC) 14. Kim WK, et al. 2006. Am. J. Pathol. 168:822. (FC) 15. Boltz A, et al. 2011. J. Biol Chem. 286:21896. PubMed
Human peripheral blood lymphocytes stained with 3G8 APC
CD16 is known as low affinity IgG receptor III (FcγRIII). It is expressed as two distinct (CD16a and CD16b) forms. CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. It is expressed on the surface of NK cells, activated monocytes, macrophages, and placental trophoblasts in humans. CD16b (FcγRIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. It is over 95% homologous to CD16a in extracellular domain and is expressed specifically on neutrophils. CD16 binds aggregated IgG or IgG-antigen complex that functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).
Other Names:
FcγRIII
Structure:
Ig superfamily, transmembrane form (50-65 kD) or GPI-linked form (48 kD)
Distribution:
NK cells, activated monocytes, macrophages, neutrophils
Function:
Low affinity IgG Fc receptor, phagocytosis, ADCC
Ligand Receptor:
Aggregated IgG, IgG-antigen complex
Antigen References:
1. Fleit H, et al. 1982. P. Natl. Acad. Sci. USA 79:3275. 2. Stroncek D, et al. 1991. Blood 77:1572. 3. Wirthmueller U, et al. 1992. J. Exp. Med. 175:1381.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-human CD16
Human peripheral blood lymphocytes stained with 3G8 APC
Biotin anti-human CD16
Human peripheral blood lymphocytes stained with biotinylated 3G8 and Sav-PE
FITC anti-human CD16
Human peripheral blood lymphocytes stained with 3G8 FITC
LEAF™ Purified anti-human CD16
Human peripheral blood lymphocytes stained with LEAF™ purified 3G8, followed by anti-mouse IgGs FITC
PE anti-human CD16
Human peripheral blood lymphocytes stained with 3G8 PE
PE/Cy5 anti-human CD16
Human peripheral blood lymphocytes stained
with 3G8 PE/Cy5
Purified anti-human CD16
Human peripheral blood lymphocytes stained with purified 3G8 and anti-mouse IgGs FITC
APC/Cy7 anti-human CD16
Human peripheral blood lymphocytes stained with 3G8 APC/Cy7
PE/Cy7 anti-human CD16
Human peripheral blood lymphocytes stained
with 3G8 PE/Cy5
Alexa Fluor® 488 anti-human CD16
Human peripheral blood lymphocytes stained with 3G8 Alexa Fluor® 488
Alexa Fluor® 647 anti-human CD16
Human peripheral blood lymphocytes stained with 3G8 Alexa Fluor® 647
Pacific Blue™ anti-human CD16
Human peripheral blood lymphocytes stained with 3G8 Pacific Blue™
Alexa Fluor® 700 anti-human CD16
Human peripheral blood lymphocytes stained with 3G8 Alexa fluor® 700
PerCP/Cy5.5 anti-human CD16
Human peripheral blood lymphocytes stained with 3G8 PerCP/Cy5.5
PerCP anti-human CD16
Human peripheral blood lymphocytes stained with 3G8 PerCP
Brilliant Violet 421™ anti-human CD16
Human peripheral blood lymphocytes were stained with CD16 (clone 3G8) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
Brilliant Violet 570™ anti-human CD16
Human peripheral blood lymphocytes were stained with CD16 (clone 3G8) Brilliant Violet 570™ (filled histogram) or mouse IgG1, κ Brilliant Violet 570™ isotype control (open histogram).
Brilliant Violet 650™ anti-human CD16
Human peripheral blood lymphocytes were stained with CD16 (clone 3G8) Brilliant Violet 650™.
