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The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Human cervical cancer cell line, Hela, stained with CD146 (clone SHM-57) APC (filled histogram) or mouse IgG2a, κ APC isotype control (open histogram).
CD146 is a 118 kD integral transmembrane glycoprotein also known as MUC18, S-Endo, MCAM, and Mel-CAM (melanoma cell adhesion molecule). It belongs to the immunoglobulin superfamily. CD146 is expressed on melanoma cells, epithelial cells, endothelial cells, fibroblasts, activated T cells, multipotent mesenchymal stromal cells, and activated keratinocytes. CD146 mediates heterophilic cell adhesion and regulates monocyte transendothelial migration. The ligand of CD146 remains to be identified.
1. Pickl WF, et al. 1997. J. Immunol. 158:2107 2. Weninger W, et al. 2000. J. Invest. Dermatol. 115:219 3. Sorrentino A, et al. 2008. Exp. Hematol. 36:1035 4. Bardin N, et al. 2009. Arterioscler. Thromb. Vasc. Biol. 29:746
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD146 (MUC18, Mel-CAM)
Human cervical cancer cell line, Hela, stained with purified SHM-57 conjugated with PE
PE anti-human CD146 (MUC18, Mel-CAM)
Human cervical cancer cell line, Hela, stained with SHM-57 PE
Human cervical cancer cell line, Hela, stained with SHM-57 Alexa Fluor® 488
PE/Cy7 anti-human CD146 (MUC18, Mel-CAM)
Human cervical cancer cell line, Hela, stained with SHM-57 PE/Cy7
APC anti-human CD146 (MUC18, Mel-CAM)
Human cervical cancer cell line, Hela, stained with CD146 (clone SHM-57) APC (filled histogram) or mouse IgG2a, κ APC isotype control (open histogram).
PerCP/Cy5.5 anti-human CD146 (MUC18, Mel-CAM)
Human cervical cancer cell line, HeLa, stained with purified CD146 (clone SHM-57) PerCP/Cy5.5 (filled histogram) or mouse IgG2a, κ PerCP/Cy5.5 isotype control (open histogram).
