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The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: Western Blot1,2, immunofluorescence microscopy1,3, immunoprecipitation1,4, block angiogenesis in vitro4,5, inhibit VE-cadherin reorganization4, and induce endothelial cell apoptosis4.
Application References:
1. Almagro S, et al. 2010. Mol. Cell Biol. 30:1703. (WB IF IP) 2. Zhang F, et al. 2004. J. Biol. Chem. 279:11760. (WB) 3. Iurlaro M, et al. 2004. Am. J. Pathol. 165:181. (IF) 4. Corada M, et al. 2001. Blood 97:1679. (IP Block) 5. Kooistra M, et al. 2005. FEBS 579:4966. (Block)
Human umbilical vein endothelial cells, HUVEC, stained with BV9 APC
CD144, also known as VE-cadherin and cadherin-5, is a 140 kD glycoprotein which is composed of 5 extracellular cadherin repeats and a highly conserved cytoplasmic tail region. It is a calcium-dependent transmembrane cell-cell adhesion molecule localized at the intercellular boundaries of endothelial cells, hematopoietic stem cells, and perineurial cells. It functions as a classic cadherin by mediating homophilic adhesion and functions as a plasma membrane attachment site for the cytoskeleton. CD144 is thought to play a role in vascular development, permeability, and remodeling.
Other Names:
VE-Cadherin, cadherin-5, CDH5
Structure:
A member of the cadherin family, is a calcium-dependent transmembrane cell-cell adhesion glycoprotein composed of 5 extracellular cadherin repeats and a highly conserved cytoplasmic tail region.
Mediates calcium-dependent homophilic cell adhesion, plays fundamental roles in microvascular permeability and in the morphogenic and proliferative events associated with angiogenesis.
Ligand Receptor:
VE-cadherin
Antigen References:
1. Taddei A, et al. 2008. Nat. Cell Biol. 10:923. 2. Gavard J, et al. 2006. Nat. Cell Biol. 8:1223. 3. Kim I, et al. 2005. Blood 106:903. 4. Suzuki S, et al. 1991. Cell Regul. 2:261.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD144 (VE-Cadherin)
Human umbilical vein endothelial cells, HUVEC, stained with purified BV9 conjugated with PE
LEAF™ Purified anti-human CD144 (VE-Cadherin)
Human umbilical vein endothelial cells, HUVEC, stained with purified BV9 conjugated with PE
PE anti-human CD144 (VE-Cadherin)
Human umbilical vein endothelial cells, HUVEC, stained with BV9 PE
APC anti-human CD144 (VE-Cadherin)
Human umbilical vein endothelial cells, HUVEC, stained with BV9 APC
