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The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Human peripheral blood monocytes were stimulated with LPS (overnight) and then stained with CD141 (clone M80) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram). Data shown was gated on the CD14+ cell population.
CD141 is a 75 kD, single chain, type I membrane glycoprotein also known as thrombomodulin, TM, THRM, THBD, and fetomodulin. CD141 is an important cofactor in the protein C anticoagulant system. After binding to its ligand thrombin, CD141 activates protein C, which degrades clotting factors Va and VIIIa, and as a consequence the amount of thrombin is reduced. CD141 is expressed on macrophages, monocytes, a subpopulation of myeloid dendritic cells, vascular endothelial cells, and keratinocytes. Besides anti-coagulation function, CD141 is also involved in embryonic and atherosclerotic plaque development.
After thrombin binding, CD141 activates protein C, which degrades clotting factors Va and VIIIa and reduces the amount of thrombin generated.
Ligand Receptor:
Thrombin.
Interaction:
Protein C, Thrombin-activatable fibrinolysis inhibitor (TAFI), Platelet factor 4 (PF4).
Antigen References:
1. Suzuki K, et al. 1987. EMBO J. 6:1891. 2. Esmon CT, et al. 1989. J. Biol. Chem. 264:4743. 3. Delvaeye M, et al. 2009. N. Engl. J. Med. 361:345. 4. Shi CS, et al. 2008. Blood 112:3661. 5. Chen LC, et al. 2009. J. Infect. 58:368.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD141 (Thrombomodulin)
Human peripheral blood monocytes stained with M80 PE
LPS-stimulated (overnight) human peripheral blood mononuclear cells stained with M80 PE (gated on CD14+ cells)
PE anti-human CD141 (Thrombomodulin)
Human peripheral blood monocytes stained with M80 PE
LPS-stimulated (overnight) human peripheral blood mononuclear cells stained with M80 PE (gated on CD14+ cells)
APC anti-human CD141 (Thrombomodulin)
Human peripheral blood monocytes were stimulated with LPS (overnight) and then stained with CD141 (clone M80) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram). Data shown was gated on the CD14+ cell population.
Biotin anti-human CD141 (Thrombomodulin)
Human peripheral blood monocytes were stained with biotinylated CD141 (clone M80, filled histogram) or mouse IgG1, κ (open histogram) isotype control, followed by Sav-PE.
