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The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application Notes:
The 18A2 monclonal antibody recognizes human CD140b also known as the platelet-derived growth factor receptor, beta polypeptide, PDGFR1, and PDGFRβ. It has been shown to be useful for flow cytometric detection of CD140b.
Application References:
1. Vogel W, et al. 2002. Haematologica 88:126.
Human PDGFRB transfected cells stained with 18A2 APC
CD140b is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. The identity of the growth factor bound to the receptor determines whether the functional receptor is a homodimer or heterodimer composed of both PDGFR-α and -β. CD140b contains two immunoglobulin-like domains and a tyrosine kinase domain with a predicted molecular weight approximately 124 kD. CD140b is widely expressed on a variety of mesenchymal-derived cells and is preferentially expressed on some tumors such as medulloblastoma. Binding of B-chain containing PDGF molecules can stimulate cell proliferation. CD140b has been shown to interact with a number of kinases (including Raf-1, NCK1, FAK, Fyn, others) as well as adaptor molecules and signaling intermediates (Crk, Grb2, Grb4, RasGAP, SHP2, SHC1, others), and has also been shown to associate with integrin β3 and nexin sorting molecules. CD140b has been implicated in several disease states including atherogenesis and oncogenesis. The PDGFRβ is heavily phosphorylated on numerous tyrosine residues through both autophosphorylation and ligand-dependent processes.
Cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. The identity of the growth factor bound to the receptor determines whether the functional receptor is a homodimer or heterodimer composed of both PDGFR1 and PD
Distribution:
Widely expressed on a variety of mesenchymal-derived cells. Preferentially expressed in medulloblastoma.
Function:
Stimulation of cell proliferation; mitogenic activity for cells of mesenchymal origin. Has been implicated in atherogenesis and oncogenesis.
Ligand Receptor:
Binds to B-chain containing PDGF molecules as well as protease-activated PDGF-C
Interacts with a number of kinases (including Raf-1, NCK1, FAK, Fyn, others) as well as adaptor molecules and signaling intermediates (Grb2, Grb4, RasGAP, SHP2, SHC1, Crk, others). Has also been shown to associate with integrin β3 and nexin sorting m
Antigen References:
1. Claesson-Welsh L, et al. 1988. Mol. Cell Biol. 8:3476. 2. Gronwald RG, et al. 1988. Proc. Natl. Acad. Sci. USA 85:3435. 3. Gilbertson DG, et al. 2001. J. Biol. Chem.276:27406. 4. Seifert RA, et al. 1989. J. Biol. Chem. 264:8771. 5. Kanakaraj P, et al. 1991. Biochemistry 30:1761.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD140b (PDGFRβ)
Human PDGFRB transfected cells stained with purified 18A2, followed by biotinylated anti-mouse IgG and Sav-PE
Biotin anti-human CD140b (PDGFRβ)
Human PDGFRB transfected cells stained with biotinylated 18A2, followed by Sav-PE
PE anti-human CD140b (PDGFRβ)
Human PDGFRB transfected cells stained with purified 18A2 PE
APC anti-human CD140b (PDGFRβ)
Human PDGFRB transfected cells stained with 18A2 APC
