The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, and immunofluorescence microscopy.
Application References:
1. McMichael, A., et al., 1987. Leucocyte Typing III. Oxford University Press. New York. 2. Knapp, W., et al., Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. 3. Schlossman, S., et al., Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Human peripheral blood monocytes stained with HCD14 APC
Description:
CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokines expression in monocytes and neutrophils.
Other Names:
LPS receptor
Structure:
GPI-linked membrane glycoprotein, 53-55 kD
Distribution:
Monocytes, macrophages, granulocytes (low)
Function:
LPS receptor, clearance of Gram-negative pathogens
Ligand Receptor:
LPS
Antigen References:
1. Stocks, S., et al., 1990. Biochem. J. 268:275. 2. Wright, S., et al., 1990. Science 249:1434.
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