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The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application Notes:
Additional reported applications (for the relevant formats of this clone) include: immunohistochemical staining in paraffin blocks of tissue sections1.
Application References:
1. Osama MA. 2010. Int. J. Clin. Exp. Pathol. 3:280. (IHC) 2. Drannik AG, et al. 2012.J. Virol. 86:4599. PubMed.
Human myeloma cell line U266 was stained with CD138 (clone DL-101) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).
CD138, a member of the syndecan protein family, is a type I integral membrane heparin sulfate proteoglycan also known as Syndecan-1. Syndecan-1 participates in cell proliferation, cell migration, and cell-matrix adhesion via interaction with collagen, fibronectin, and other soluble molecules (such as FGF-basic). It is expressed on normal and malignant human plasma cells, pre-B cells, epithelial cells, and endothelial cells.
Other Names:
Syndecan-1, B-B4
Structure:
100-200 kD type I integral transmembrane glycoprotein
Adhesion, control cell morphology, regulate cell growth
Ligand Receptor:
FGFb, collagen, fibronectin
Antigen References:
1. Sanderson RD, et al. 1992. Cell. Regul. 1:27. 2. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules: The CD Markers Wiley-Liss A John Wiley & Sons Inc, Publication.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 were stained with CD138 (clone DL-101) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
FITC anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with CD138 (clone DL-101) FITC (filled histogram) or mouse IgG1, κ FITC isotype control (open histogram).
PE anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with CD138 (clone DL-101) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
APC anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with CD138 (clone DL-101) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).
PerCP/Cy5.5 anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with CD138 (clone DL-101) PerCP/Cy5.5 (filled histogram) or mouse IgG1, κ PerCP/Cy5.5 isotype control (open histogram).
