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APC anti-human CD11a Antibody
Cat. # Size Price  

301212 100 tests $265.00
    
Product Details

Clone: HI111 (See other available formats)
Isotype: Mouse IgG1, κ
Isotype Control:APC Mouse IgG1, κ Isotype Ctrl
Workshop
Number:
IV N231
Reactivity: Human, Cross-Reactivity: Chimpanzee, Baboon, Capuchin Monkey, Horse (Equine), Cattle (Bovine, Cow), Sheep (Ovine), Dog (Canine), Rabbit (Lapine)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
Excitation
Laser:
Red Laser (633 nm)
Application
Notes:
Clone HI111 epitope maps to the top region of the I domain that is close to the putative ligand-binding site surrounding the MIDAS (metal ion-dependent adhesion site). HI111 is specific for the closed confirmation of the integrin.8 Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, Western blotting2, and blocking of cell-cell interaction and inhibition the binding of ICAM-14. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 301214).
Application
References:

  Publication Library
 Image 1
Human peripheral blood lymphocytes stained with HI111 APC


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See APC spectral data





 


Antigen Details

Description: CD11a is a 170-180 kD type I transmembrane glycoprotein also known as LFA-1α chain and integrin αL subunit. CD11a non-covalently associates with integrin β2 (CD18) to form LFA-1. It is expressed on all leukocytes, including B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils. It is absent on non-hematopoietic tissues and platelets. CD11a plays a central role in leukocyte cell-cell interactions and is important in lymphocyte costimulation. CD11a/CD18 binds to ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50).
Other Names: LFA-1α chain, Integrin αL subunit, αL Integrin, ITGAL
Structure: Integrin, type I transmembrane glycoprotein, noncovalently linked with integrin β2 (CD18) forms LFA-1, 170-180 kD
Distribution: Leukocytes
Function: Adhesion, costimulation
Ligand Receptor: ICAM-1(CD54), ICAM-2(CD102), ICAM-3(CD50)
Antigen
References:
1. Lub M, et al. 1995. Immunol. Today 16:479.
2. Parsons J. 1996. Curr. Opin. Cell Biol. 8:146.
GeneID: 3683
UniProt: View information about CD11a on UniProt.org
Keywords: APC anti-human CD11a, HI111, APC, LFA-1α chain, Integrin αL subunit, αL Integrin, ITGAL, Human, Cross-Reactivity: Chimpanzee, Baboon, Capuchin Monkey, Horse (Equine), Cattle (Bovine, Cow), Sheep (Ovine), Dog (Canine), Rabbit (Lapine), Flow Cytometry, Immunology, Antibodies
Technical Data Sheet (pdf)
Material Safety Data Sheet
Certificate of Analysis
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Version: 3 Revision Date: 2014-08-19
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • APC anti-human CD11a
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HI111 APC

  • Biotin anti-human CD11a
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with biotinylated HI111, followed by Sav-PE

  • FITC anti-human CD11a
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HI111 FITC

  • LEAF™ Purified anti-human CD11a
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with LEAF™ purified HI111, followed by anti-mouse IgGs FITC

  • PE anti-human CD11a
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HI111 PE

  • PE/Cy5 anti-human CD11a
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HI111 PE/Cy5

  • Purified anti-human CD11a
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with purified HI111, followed by anti-mouse IgGs FITC

  • Alexa Fluor® 488 anti-human CD11a
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HI111 Alexa Fluor® 488

  • Alexa Fluor® 647 anti-human CD11a
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HI111 Alexa Fluor® 647

  • PE/Cy7 anti-human CD11a
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained CD11a (clone HI111) PE/Cy7 (filled histogram) or mouse IgG1, κ PE/Cy7 isotype control (open histogram).

  • Alexa Fluor® 594 anti-human CD11a
    Human neutrophils were fixed with

    Human neutrophils were fixed with 1% paraformaldehyde (PFA), then stained with 5 µg/ml anti-human CD11a (clone HI111) Alexa Fluor® 594 (red). Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.

    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD11a (clone HI111) Alexa Flour® 594 (filled histogram) or mouse IgG1, κ Alexa Flour™ 594 isotype control (open histogram). The data was acquired by BD LSRFortessa™ cell analyzer equipped with the Yellow-Green Laser (561 nm).

  • Purified anti-human CD11a (MaxPar® Ready)
    Human PBMCs stained with 154Sm-anti-CD45

    Human PBMCs stained with 154Sm-anti-CD45 (HI30) and 142Nd-anti-CD11a (HI111). Data provided by DVS Sciences.

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