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The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: Western blotting and ELISA.
Application References:
1. Ozeki M, et al. 2008. J. Leukocyte Biol. 84:769. (FC) PubMed
Human peripheral blood lymphocytes stained with 2M2 APC
β2-microglobulin (β2M) is a 12 kD nonpolymorphic Ig like protein. It is a non-membrane-anchored glycoprotein and is noncovalently associated with 39-44 kD polymorphic heavy chains of MHC class I molecules to form HLA class I antigen complex. In association with HLA class I, β2M is expressed on all leukocytes, platelets, endothelial cells and epithelial cells. β2M plays an essential role both in governing MHC class I molecules' stability and in promoting antigen binding and presenting the antigen to CD3/TCR complex of CD8+ T cells.
Other Names:
β2M, β2-M, beta2-microglobulin
Structure:
Nonpolymorphic Ig like structure, noncovalently associated with heavy chain of MHC class I molecules, 12 kD
Govern MHC class I molecule stability, promote MHC class I molecules binding and presenting peptide antigens to CD8+ T cells
Ligand Receptor:
CD3/TCR complex, CD8
Antigen References:
1. Engelhard VH. 1994. Curr. Opin. Immunol. 6:13. 2. Williams DB, et al. 1989. J. Immunol. 142:2796. 3. Danliczyk UG and Delovitch TL. 1994. J. Immunol. 153:3533. 4. Williams A, et al. 2002. Tissue Antigens 59:3.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human β2-microglobulin
Human peripheral blood lymphocytes stained with purified 2M2, followed by anti-mouse FITC
FITC anti-human β2-microglobulin
Human peripheral blood lymphocytes stained with 2M2 FITC
PE anti-human β2-microglobulin
Human peripheral blood lymphocytes stained with 2M2 PE
Biotin anti-human β2-microglobulin
Human peripheral blood lymphocytes stained with biotinylated 2M2, followed by Sav-PE
Pacific Blue™ anti-human β2-microglobulin
Human peripheral blood lymphocytes stained with 2M2 Pacific Blue™
APC anti-human β2-microglobulin
Human peripheral blood lymphocytes stained with 2M2 APC
