Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 1.0 µg per million cells in a volume of 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® 647 is a registered trademark of Molecular Probes, Inc. Alexa Fluor® 647 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
1. Szabo, S,J., et al. 2000. Cell 100:655 (ICFC, WB) 2. Hwang, E.S., et al. 2005. J Exp Med 202:1289 (ICFC, WB, IP)
PMA/ionomycin-stimulated (6 hours) human peripheral blood lymphocytes intracellularly stained with IFN-γ (4S.B3) FITC and 4B10 Alexa Fluor® 647
Description:
T-bet, also known as T-box transcription factor T-bet, is considered to be a "master regulator" of Th1 lymphoid development controlling the production of the cytokine IFN-γ. T-bet is widely expressed in hematopoietic cells including stem cells, NK cells, B cells, and T cells. T-bet is critical for the control of microbial pathogens and knockout animals show multiple physiologic and inflammatory features characteristic of asthma. T-bet expression is optimally observed after IL-12 stimulation and can be suppressed by addition of the Th2 cytokine IL-4 or neutralization of IL-12.
Other Names:
T box expressed in T cells, T box 21, TBLYM
Structure:
T-box transcription factor, approximately 58 kD
Distribution:
Nuclear; expressed in T cells, hematopoietic stem cells, NK cells, B cells, lung, spleen.
Function:
Th1-specific T-box transcription factor controlling expression of the hallmark Th1 cytokine, interferon gamma (IFN-γ). T-bet expression is critical for the control of microbial pathogens.
Antigen References:
1. Szabo, S.J., et al. 2000. Cell 100:655. 2. Szabo, S.J., et al. 2002. Science 295:338. 3. Finotto, S., et al. 2002. Science 295:336. 4. Mullen, A.C., et al. 2001. Science 292:1907.
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