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Anti-DYKDDDDK Tag (L5) Affinity Gel
Anti-DYKDDDDK Tag (L5) Affinity Gel
651501 500 µl $175.00     
651502 2.0 ml $385.00     
651503 5.0 ml $695.00     
Product Details
Clone: L5
Reactivity: DYKDDDDK tag epitope
Immunogen: Anti-DYKDDDDK tag
Formulation: 50% anti-DYKDDDDK-tag (L5) antibody conjugated resin is supplied in 1X PBS and 0.09% NaN3.
Storage & Handling: Upon receipt, store at 4°C. The unopened product is stable for one year upon arrival.
Application:

IP, Purification of DYKDDDDK-tagged fusion proteins from cell lysates - Quality tested
Recommended Usage:

Resin binding capacity is greater than 0.8 mg/mL.
COA:
Enter Lot#:   
Application
Notes:

The optimal usage should be determined by titration for each individual assay of interest. For a detailed protocol on using the affinity gel for protein purification from cell lysates, download our Technical Bulletin.
Anti-DYKDDDDK tag affinity resin (L5-agarose)
Anti-DYKDDDDK tag affinity resin (L5-agarose) retains binding capacity even after 10 rounds of purification and elution.

SDS PAGE gel image of
SDS PAGE gel image of protein purified from the 1st and 10th rounds by either L5 agarose or competitor's equivalent product.


Compare all formats



Description:

The anti-DYKDDDDK tag affinity gel is composed of the L5 antibody conjugated to an agarose resin, which can be used for purification of proteins expressing the octapeptide, DYKDDDDK.  The DYKDDDDK tag, commonly referred to as Sigma®'s FLAG® Tag, is often used as a protein modification in order to simplify the labeling and detection of proteins. The binding capacity of the L5 agarose resin is greater than 0.8 mg/ml.  The resin can be re-used at least five times (see technical bulletin for regeneration and storage). This affinity gel is also useful for immunoprecipitation of DYKDDDDK-tagged protein.
Other Names: Anti-DYKDDDDK-tag (L5) antibody conjugated agarose resin
GeneID: NA
Latest Publications: View the latest DYKDDDDK articles on HighwirePress.com
UniProt: View information about DYKDDDDK on UniProt.org
Keywords: Anti-DYKDDDDK Tag (L5) Affinity Gel, L5, Purified, Anti-DYKDDDDK-tag (L5) antibody conjugated agarose resin , DYKDDDDK tag epitope, Immunology, Antibodies
Related Products
DescriptionCloneApplications
Purified anti-DYKDDDDK TagL5WB, IP, IF, IHC, ELISA, FC
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
  • Purified anti-DYKDDDDK Tag
    Cell extracts from untransfected 293T

    Cell extracts from untransfected 293T cells (lane 1) or 293T cells transfected with a plasmid encoding DYKDDDDK-tagged protein (lane 2), using anti-DYKDDDDK, clone L5.

  • Anti-DYKDDDDK Tag (L5) Affinity Gel
    Anti-DYKDDDDK tag affinity resin (L5-agarose)

    Anti-DYKDDDDK tag affinity resin (L5-agarose) retains binding capacity even after 10 rounds of purification and elution.

    SDS PAGE gel image of

    SDS PAGE gel image of protein purified from the 1st and 10th rounds by either L5 agarose or competitor's equivalent product.

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