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Annexin V Binding Buffer is recommended for use with Annexin V reagents. Cells should be resuspended in Annexin V Binding Buffer at a concentration 1x106/ml and then combined with Annexin V reagents at the recommended volumes.
COA:
Application Notes:
Annexin V Staining 1. Wash cells twice with cold BioLegend cell staining buffer (cat # 420201) and then resuspend cells in Annexin V Binding Buffer (cat # 422201) at a concentration of 1x106 cells/ml. 2. Transfer 100 µl of cell suspension in 5 ml test tube. 3. Add 5 µl of fluorochrome conjugated Annexin V. 4. Add 10 µl of PI solution (cat # 421301) or 7-AAD (cat # 420401). 5. Gently vortex the cells and incubate for 15 min at RT (25 °C) in the dark. 6. Add 400 µl of Annexin V Binding Buffer (cat # 422201) to each tube. Analyze by flow cytometry.
Application References:
Vermes I, et al. 1995. J. Immunol. Methods 184:39.
Description:
Annexin V Binding Buffer has been formulated for flow cytometric labeling of apoptotic cells with Annexin V reagents. The buffer is provided as a ready-to-use solution.
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