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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 700 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 700.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
This reagent is developed for immunofluorescent staining for flow cytometric analysis, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633nm / 635nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
The RB6-8C5 antibody has been used to identify peripheral blood neutrophils and deplete granulocytes in vivo. Additional reported applications (for relevant formats of this clone) include: in vitro complement-mediated cytotoxicity2, in vivo depletion3-5,9, immunoprecipitation1, immunohistochemical staining6 (including paraffin embedded sections9,16, acetone-fixed frozen sections11 and zinc-fixed15 sections), and Western blotting7.
Special Note: The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 108414). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 108436) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Application References:
1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (IP) 2. Brummer E, et al. 1984. J. Leukocyte Biol. 36:505. (C') 3. Stoppacciaro A, et al. 1993. J. Exp. Med. 178:151. (Deplete) 4. Tumpey TM, et al. 1996. J. Virol. 70:898. (Deplete) 5. Czuprynski CJ, et al. 1994. J. Immunol. 152:1836. (Deplete) 6. Nitta H, et al. 1997. Cell Vision 4:73. (IHC) 7. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (WB) 8. Engwerda CR, et al. 2004. Am. J. Pathol. 165:2123. 9. Brown CR, et al. 2004. Infect. Immun. 72:4956. (Deplete, IHC) 10. Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC) PubMed 11. Li M, et al. 2006. P. Natl. Acad. Sci USA 103:11736. (IHC) 12. Dzhagalov I, et al. 2007. Blood 109:1620. (FC) PubMed 13. Fazilleau N, et al. 2007. Nature Immunol. 8:753. (FC) PubMed 14. Heuser M, et al. 2007. Blood 110:1639. (FC) PubMed 15. Wang T, et al. 2007. Infect. Immun. 75:1144. (IHC) 16. Bosio CM, et al. 2007. J. Immunol. 178:4538. (IHC) 17. Boehme SA, et al. 2009. Int. Immunol. 21:81. (IHC)
C57BL/6 mouse bone marrow (gated on myeloid cell population) stained with Ly-6G/Ly-6C (clone RB6-8C5) Alexa Fluor® 700 (filled histogram) or rat IgG2b, κ Alexa Fluor® 700 isotype control (open histogram).
Gr-1 is a 21-25 kD protein also known as Ly-6G/Ly-6C. This myeloid differentiation antigen is a glycosylphosphatidylinositol (GPI)-linked protein expressed on granulocytes and macrophages. In bone marrow, the expression levels of Gr-1 directly correlate with granulocyte differentiation and maturation; Gr-1 is also transiently expressed on bone marrow cells in the monocyte lineage. Immature Myeloid Gr-1+ cells play a role in the development of antitumor immunity.
Other Names:
Gr-1
Structure:
21-25 kD
Distribution:
Granulocytes, monocytes
Antigen References:
1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. 2. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. 3. Goni O, et al. 2002. Int. Immunol. 14:1125.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-mouse Ly-6G/Ly-6C (Gr-1)
C57BL/6 mouse bone marrow stained with Ly-6G/Ly-6C (clone RB6-8C5) APC and CD11b PE.
Biotin anti-mouse Ly-6G/Ly-6C (Gr-1)
C57BL/6 mouse bone marrow stained with biotinylated Ly-6G/Ly-6C (clone RB6-8C5) and CD11b PE.
FITC anti-mouse Ly-6G/Ly-6C (Gr-1)
C57BL/6 mouse bone marrow stained with Ly-6G/Ly-6C (clone RB6-8C5) FITC and CD11b PE.
LEAF™ Purified anti-mouse Ly-6G/Ly-6C (Gr-1)
C57BL/6 mouse bone marrow cells were stained with LEAF™ purified Ly-6G/Ly-6C (clone RB6-8C5) (filled histogram) or rat IgG2b, κ isotype control (open histogram), followed by anti-rat IgG FITC. Data shown was gated on myeloid cell population.
PE anti-mouse Ly-6G/Ly-6C (Gr-1)
C57BL/6 mouse bone marrow cells were stained with Ly-6G/Ly-6C (clone RB6-8C5) PE (filled histogram) or rat IgG2b, κ PE isotype control (open histogram). Data shown was gated on myeloid cell population.
PE/Cy5 anti-mouse Ly-6G/Ly-6C (Gr-1)
C57BL/6 bone marrow cells stained with RB6-8C5 FITC and CD11b PE
Purified anti-mouse Ly-6G/Ly-6C (Gr-1)
C57BL/6 mouse bone marrow cells were stained with purified Ly-6G/Ly-6C (clone RB6-8C5) (filled histogram) or rat IgG2b, κ isotype control (open histogram), followed by anti-rat IgG FITC. Data shown was gated on myeloid cell population.
PE/Cy7 anti-mouse Ly-6G/Ly-6C (Gr-1)
C57BL/6 mouse bone marrow cells were stained with Ly-6G/Ly-6C (clone RB6-8C5) PE/Cy7 (filled histogram) or rat IgG2b, κ PE/Cy7 isotype control (open histogram). Data shown was gated on myeloid cell population.
Alexa Fluor® 488 anti-mouse Ly-6G/Ly-6C (Gr-1)
C57BL/6 mouse bone marrow (gated on myeloid cell population) stained with Ly-6G/Ly-6C (clone RB6-8C5) Alexa Fluor® 488 (filled histogram) or rat IgG2b, κ Alexa Fluor® 488 isotype control (open histogram).
Alexa Fluor® 647 anti-mouse Ly-6G/Ly-6C (Gr-1)
C57BL/6 mouse bone marrow (gated on myeloid cell population) stained with Ly-6G/Ly-6C (clone RB6-8C5) Alexa Fluor® 647 (filled histogram) or rat IgG2b, κ Alexa Fluor® 647 isotype control (open histogram).
Alexa Fluor® 700 anti-mouse Ly-6G/Ly-6C (Gr-1)
C57BL/6 mouse bone marrow (gated on myeloid cell population) stained with Ly-6G/Ly-6C (clone RB6-8C5) Alexa Fluor® 700 (filled histogram) or rat IgG2b, κ Alexa Fluor® 700 isotype control (open histogram).
APC/Cy7 anti-mouse Ly-6G/Ly-6C (Gr-1)
C57BL/6 mouse bone marrow (gated on myeloid cell population) stained with Ly-6G/Ly-6C (clone RB6-8C5) APC/Cy7 (filled histogram) or rat IgG2b, κ APC/Cy7 isotype control (open histogram).
Pacific Blue™ anti-mouse Ly-6G/Ly-6C (Gr-1)
C57BL/6 mouse bone marrow (gated on myeloid cell population) stained with Ly-6G/Ly-6C (clone RB6-8C5) PB (red histogram) or rat IgG2b, κ PB isotype control (green histogram).
PerCP/Cy5.5 anti-mouse Ly-6G/Ly-6C (Gr-1)
C57BL/6 mouse bone marrow (gated on myeloid cell population) stained with Ly-6G/Ly-6C (clone RB6-8C5) PerCP/Cy5.5 (filled histogram) or rat IgG2b, κ PerCP/Cy5.5 isotype control (open histogram).
PerCP anti-mouse Ly-6G/Ly-6C (Gr-1)
C57BL/6 mouse bone marrow (gated on myeloid cell population) stained with Ly-6G/Ly-6C (clone RB6-8C5) PerCP (filled histogram) or rat IgG2b, κ PerCP isotype control (open histogram).
C57BL/6 mouse bone marrow cells were stained with Ly-6G/Ly-6C (clone RB6-8C5) Brilliant Violet 421™ (filled histogram) or rat IgG2b, κ Brilliant Violet 421™ isotype control (open histogram). Data shown was gated on myeloid cell population.
C57BL/6 mouse bone marrow cells were stained with Ly-6G/Ly6C (clone RB6-8C5) Brilliant Violet 570™ (filled histogram) or rat IgG2b, κ Brilliant Violet 570™ isotype control (open histogram). Data shown was gated on myeloid cell population.
C57BL/6 mouse bone marrow cells were stained with LEAF™ purified Ly-6G/Ly-6C (clone RB6-8C5) (filled histogram) or rat IgG2b, κ isotype control (open histogram), followed by anti-rat IgG FITC. Data shown was gated on myeloid cell population.
