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The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 700.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
This reagent is developed for immunofluorescent staining for flow cytometric analysis; the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Alexa Fluor® 700 is a registered trademark of Molecular Probes, Inc. Alexa Fluor® 700 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Additional reported applications (for the relevant formats) include: depletion and immunohistochemistry9. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 127620). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 127632) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Application References:
1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC) 2. Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC) 3. Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC) 4. Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed 5. Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed 6. Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed 7. Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed 8. Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed 9. Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC)
C57BL/6 bone marrow cells stained with 1A8 Alexa Fluor® 700
Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.
Other Names:
Ly6G
Structure:
A 21-35 kD GPI-anchorded membrane protein
Distribution:
Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse Ly-6G
C57BL/6 bone marrow cells stained with 1A8 purified, followed by anti-rat IgG PE (myeloid cells were gated for analysis)
Biotin anti-mouse Ly-6G
C57BL/6 bone marrow cells stained with 1A8 biotin, followed by Sav-PE (gated on myeloid cells)
FITC anti-mouse Ly-6G
BALB/cmouse bone marrow cells stained with 1A8 FITC (gated on myeloid cells)
PE anti-mouse Ly-6G
C57BL/6 bone marrow cells stained with 1A8 PE (gated on myeloid cells)
Alexa Fluor® 647 anti-mouse Ly-6G
C57BL/6 mouse bone marrow cells stained with 1A8 Alexa Fluor® 647
Pacific Blue™ anti-mouse Ly-6G
C57BL/6 bone marrow cells stained with 1A8 Pacific Blue™
APC anti-mouse Ly-6G
C57BL/6 bone marrow cells stained with 1A8 APC
PerCP/Cy5.5 anti-mouse Ly-6G
C57BL/6 bone marrow cells stained with 1A8 PerCP/Cy5.5
PE/Cy7 anti-mouse Ly-6G
C57BL/6 bone marrow cells stained with 1A8 PE/Cy7
LEAF™ Purified anti-mouse Ly-6G
C57BL/6 bone marrow cells stained with 1A8 purified, followed by anti-rat IgG PE (myeloid cells were gated for analysis)
Alexa Fluor® 700 anti-mouse Ly-6G
C57BL/6 bone marrow cells stained with 1A8 Alexa Fluor® 700
APC/Cy7 anti-mouse Ly-6G
C57BL/6 bone marrow cells stained with 1A8 APC/Cy7
Brilliant Violet 421™ anti-mouse Ly-6G
C57BL/6 mouse bone marrow cells were stained with Ly-6G (clone 1A8) Brilliant Violet 421™ (filled histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram). Data shown was gated on myeloid cell population.
Brilliant Violet 570™ anti-mouse Ly-6G
C57BL/6 bone marrow cells were stained with Ly-6G (clone 1A8) Brilliant Violet 570™ (filled histogram) or rat IgG2a, κ Brilliant Violet 570™ isotype control (open histogram). Data shown was gated on myeloid cell population.
Ultra-LEAF™ Purified anti-mouse Ly-6G
C57BL/6 bone marrow cells stained with 1A8 purified, followed by anti-rat IgG PE (myeloid cells were gated for analysis)
