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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 700 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 700.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
This reagent is developed for immunofluorescent staining for flow cytometric analysis, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633nm / 635nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for the relevant formats) include: in vivo and in vitro depletion1,2,7, costimulation of CD3/TCR-mediated signal transduction3,4, and immunohistochemical staining5 of acetone-fixed frozen sections. The 30-H12 antibody does not react with Thy-1.1 alloantigen of the AKR/J and PL strains. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302) is recommended prior to immunofluorescent staining. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 105310).
Application References:
1. Hathcock KS. 1991. Current Protocols in Immunology. 3.4.1. (Deplete) 2. Seaman WE. 1983. J. Immunol. 130:1713. (Deplete) 3. Nakashima I, et al. 1991. J. Immunol. 147:1153. (Costim) 4. Nakashima I, et al. 1993. J. Immunol. 151:3511. (Costim) 5. Ledbetter JA, et al. 1980. J. Exp. Med. 152:280. (IHC) 6. Hardy B, et al. 2005. Int. Immunol. 17:615. 7. Drobyski W, et al. 1996. Blood 87:5355. (Deplete) 8. Dyer KD, et al. 2007. J. Immunol. 179:1693. (FC) PubMed
C57BL/6 mouse thymocytes stained with 30-H12 Alexa Fluor® 700
CD90.2 is a 25-35 kD immunoglobulin superfamily member also known as Thy1.2. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells in Thy1.2 bearing mouse strains (Balb/c, CBA/J, C3H/He, C57BL/-, DBA, NZB/-). CD90.2 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90.2 is involved in costimulation of lymphocyte proliferation and induction of hematopoietic stem cells differentiation. CD90.2 has been shown to interact with CD45. The 30-H12 antibody has been reported to induce Ca2+ flux in thymocytes and, in combination with antibody against the CD3/TCR complex, promote thymocyte apoptosis and inhibit CD3-mediated proliferative responses of mature T lymphocytes.
Other Names:
Thy-1.2
Structure:
Ig superfamily, 25-35 kD
Distribution:
Hematopoietic stem cells and neurons, all thymocytes, peripheral T cells of the Thy-1.2 bearing mice
Function:
Lymphocyte costimulation, proliferation and differentiation of hematopoietic stem cells
Ligand Receptor:
CD45
Antigen References:
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Craig W, et al. 1993. J. Exp. Med. 177:1331. 3. Reif AE and Schlesinger M. 1989. Cell Surface Antigen Thy-1. 4. Mayani H, et al. 1994. Blood 83:2410.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-mouse CD90.2
C57BL/6 mouse thymocytes stained with biotinylated 30-H12, followed by Sav-PE
FITC anti-mouse CD90.2
C57BL/6 thymocytes stained with 30-H12 FITC
LEAF™ Purified anti-mouse CD90.2
C57BL/6 mouse thymocytes stained with LEAF™ purified 30-H12, followed by anti-rat IgG FITC
PE anti-mouse CD90.2
C57BL/6 mouse thymocytes stained with 30-H12 PE
Purified anti-mouse CD90.2
C57BL/6 mouse thymocytes stained with purified 30-H12, followed by anti-rat IgG FITC
APC anti-mouse CD90.2
C57BL/6 mouse thymocytes stained with 30-H12 APC
PE/Cy5 anti-mouse CD90.2
C57BL/6 mouse thymocytes stained with 30-H12 PE/Cy5
Alexa Fluor® 488 anti-mouse CD90.2
C57BL/6 mouse thymocytes stained with 30-H12 Alexa Fluor® 488
Alexa Fluor® 647 anti-mouse CD90.2
C57BL/6 mouse thymocytes stained with 30-H12 Alexa Fluor® 647
Alexa Fluor® 700 anti-mouse CD90.2
C57BL/6 mouse thymocytes stained with 30-H12 Alexa Fluor® 700
PerCP anti-mouse CD90.2
C57BL/6 splenocytes stained with purified 30-H12 PerCP
Pacific Blue™ anti-mouse CD90.2
Balb/c mouse thymocytes stained with 30-H12 Pacific Blue™
PE/Cy7 anti-mouse CD90.2
C57BL/6 splenocytes stained with 30-H12 PE/Cy7
APC/Cy7 anti-mouse CD90.2
C57BL/6 splenocytes stained with 30-H12 APC/Cy7
Brilliant Violet 570™ anti-mouse CD90.2
C57BL/6 splenocytes were stained with CD90.2 (clone 30-H12) Brilliant Violet 570™ (filled histogram) or rat IgG2b, κ Brilliant Violet 570™ isotype control (open histogram).
