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The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 700.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
This reagent is developed for immunofluorescent staining for flow cytometric analysis; the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Alexa Fluor® 700 is a registered trademark of Molecular Probes, Inc. Alexa Fluor® 700 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
The HM48-1 antibody is useful for blocking in vitro and in vivo CD48 mediated interactions. Additional reported applications (for the relevant formats) include: immunoprecipitation1, costimulation of T cell proliferation1,2, blocking of CD48-CD2 interaction1, and inhibition of CTL activity and graft rejection1,2. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 103408). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 103430) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Application References:
1. Kato K, et al. 1992. J. Exp. Med. 176:1241. (IP, Costim, Block) 2. Qin L, et al. 1994. J. Exp. Med. 179:341. (Costim, Block)
C57BL/6 splenocytes stained with HM48-1 Alexa Fluor® 700
CD48 is a 45 kD GPI-anchored glycoprotein also known as BCM1, Blast-1 (human), and OX-45 (rat). It is a member of the Ig superfamily, expressed on T and B cells and monocytes/macrophages. It plays a role in adhesion and T cell recognition. The primary ligands for CD48 are CD2 and CD244.
Other Names:
BCM1, Blast-1 (human), OX-45 (rat)
Structure:
Ig superfamily, 45 kD
Distribution:
T and B cells, monocytes/macrophages
Function:
Adhesion, T cell recognition
Ligand Receptor:
CD2, CD244
Antigen References:
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Flament C, et al. 1996. Hum. Immunol. 46:82. 3. Van der Merwe PA, et al. 1995. Curr. Biol. 5:74. 4. Latchman Y, et al. 1998. J. Immunol. 161:5809.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
FITC anti-mouse CD48
C57BL/6 splenocytes stained with
HM48-1 FITC
LEAF™ Purified anti-mouse CD48
C57BL/6 mouse splenocytes stained with LEAF™ purified HM48-1, followed by anti-Armennian hamster IgG FITC
PE anti-mouse CD48
C57BL/6 mouse splenocytes stained with HM48-1 PE
Purified anti-mouse CD48
C57BL/6 mouse splenocytes stained with purified HM48-1, followed by anti-Armenian hamster IgG FITC
Biotin anti-mouse CD48
C57BL/6 mouse splenocytes stained with biotinylated HM48-1, followed by Sav-PE
APC anti-mouse CD48
C57BL/6 mouse splenocytes stained with HM48-1 APC
Alexa Fluor® 488 anti-mouse CD48
C57BL/6 mouse splenocytes stained with HM48-1 Alexa Fluor® 488
Alexa Fluor® 647 anti-mouse CD48
C57BL/6 mouse splenocytes stained with HM48-1 Alexa Fluor® 647
Pacific Blue™ anti-mouse CD48
C57BL/6 mouse splenocytes stained with Pacific blue™ HM48-1
PE/Cy5 anti-mouse CD48
C57BL/6 splenocytes stained with HM48-1 PE/CY5
PerCP/Cy5.5 anti-mouse CD48
C57BL/6 mouse splenocytes stained with HM48-1 PerCP/Cy5.5
PE/Cy7 anti-mouse CD48
C57BL/6 splenocytes stained with HM48-1 PE/Cy7
Alexa Fluor® 700 anti-mouse CD48
C57BL/6 splenocytes stained with HM48-1 Alexa Fluor® 700
Brilliant Violet 421™ anti-mouse CD48
C57BL/6 mouse splenocytes were stained with CD48 (clone HM48-1) Brilliant Violet 421™ (filled histogram) or Armenian hamster IgG Brilliant Violet 421™ isotype control (open histogram).
Ultra-LEAF™ Purified anti-mouse CD48
C57BL/6 mouse splenocytes stained with LEAF™ purified HM48-1, followed by anti-Armennian hamster IgG FITC
