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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 700 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 700.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
This reagent is developed for immunofluorescent staining for flow cytometric analysis, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633nm / 635nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
CD56 is a single transmembrane glycoprotein also known as NCAM (Neural Cell Adhesion Molecule), Leu-19, or NKH1. It is a member of the Ig superfamily. The 140 kD isoform is expressed on NK cells and NK-T cells. CD56 is also expressed in the brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias also express CD56. CD56 plays a role in homophilic and heterophilic adhesion via binding to itself or heparin sulfate.
Other Names:
Leu-19, NKH1
Structure:
Ig superfamily, single transmembrane or GPI-anchored glycoprotein
Distribution:
NK cells, T subset, neural tissue, some LGL and myeloid leukemias
Function:
Adhesion
Ligand Receptor:
Heparin sulfate
Antigen References:
1. Lanier L, et al. 1991. J. Immunol. 146:4421. 2. Hemperly J, et al. 1990. J. Mol. Neurosci. 2:71. 3. Cremer H, et al. 1994. Nature 367:455.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD56 (NCAM)
Human peripheral blood lymphocytes stained with purified HCD56,then detected with anti-mouse IgG FITC
FITC anti-human CD56 (NCAM)
Human peripheral blood lymphocytes stained with HCD56 FITC
PE anti-human CD56 (NCAM)
Human peripheral blood lymphocytes stained with HCD56 PE
PE/Cy5 anti-human CD56 (NCAM)
Human peripheral blood lymphocytes stained with HCD56 PE/Cy5
APC anti-human CD56 (NCAM)
Human peripheral blood lymphocytes stained with HCD56 APC
Alexa Fluor® 488 anti-human CD56 (NCAM)
Human peripheral blood lymphocytes stained with HCD56 Alexa Fluor® 488
Alexa Fluor® 647 anti-human CD56 (NCAM)
Human NK-92 cells were stained with CD56 (clone HCD56) Alexa Fluor® 647. Cells were imaged with a Zeiss Axio Observer Z1 spinning disc confocal, average exposure time ~0.23 seconds. Credit: Dr. Jordan Orange and Dr. Emily Mace, University of Pennsylvania School of Medicine Children's Hospital of Philadelphia, Division of Immunology.
Human peripheral blood lymphocytes stained with HCD56 Alexa Fluor® 647
Alexa Fluor® 700 anti-human CD56 (NCAM)
Human peripheral blood lymphocytes stained with CD16 (3G8) PE and HCD56 Alexa Fluor® 700
PE/Cy7 anti-human CD56 (NCAM)
Human peripheral blood lymphocytes stained with HCD56 PE/Cy7
Biotin anti-human CD56 (NCAM)
Human NK-92 cells were stained with CD56 (clone HCD56) Biotin, and then secondarily stained with Streptavidin-Alexa Fluor® 488. Cells were imaged with a Zeiss Axio Observer Z1 spinning disc confocal, average exposure time ~0.23 seconds. Credit: Dr. Jordan Orange and Dr. Emily Mace, University of Pennsylvania School of Medicine Children's Hospital of Philadelphia, Division of Immunology.
Human peripheral blood lymphocytes stained with biotinylated HCD56, followed by Sav-PE
PerCP/Cy5.5 anti-human CD56 (NCAM)
Human peripheral blood lymphocytes stained with HCD56 PerCP/Cy5.5
LEAF™ Purified anti-human CD56 (NCAM)
Human peripheral blood lymphocytes stained with LEAF™ purified HCD56, followed by anti-mouse IgG FITC
Pacific Blue™ anti-human CD56 (NCAM)
Human peripheral blood lymphocytes stained with HCD56 Pacific Blue™
APC/Cy7 anti-human CD56 (NCAM)
Human peripheral lymphocytes were stained with CD16 FITC and CD56 (clone HCD56) APC/Cy7 (top) or mouse IgG1 APC/Cy7 isotype control (bottom).
Brilliant Violet 421™ anti-human CD56 (NCAM)
Human peripheral blood lymphocytes were stained with CD3 FITC and CD56 (clone HCD56) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
Brilliant Violet 570™ anti-human CD56 (NCAM)
Human peripheral blood lymphocytes were stained with CD3 FITC and CD56 (clone HCD56) Brilliant Violet 570™ (top) or mouse IgG1, κ Brilliant Violet 570™ isotype control (bottom).
Brilliant Violet 605™ anti-human CD56 (NCAM)
Human peripheral blood lymphocytes were stained with CD3 FITC and CD56 (clone HCD56) Brilliant Violet 605™.
Brilliant Violet 711™ anti-human CD56 (NCAM)
Human peripheral blood lymphocytes were stained with CD3 FITC and CD56 (clone HCD56) Brilliant Violet 711™.
Brilliant Violet 510™ anti-human CD56 (NCAM)
Human peripheral blood lymphocytes were stained with CD3 APC and CD56 (clone HCD56) Brilliant Violet 510™.
PerCP anti-human CD56 (NCAM)
Human peripheral blood lymphocytes were stained with CD56 (clone HCD56) PerCP (filled histogram) or mouse IgG1, κ PerCP isotype control (open histogram).
