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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 700 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 700.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
This reagent is developed for immunofluorescent staining for flow cytometric analysis; the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Alexa Fluor® 700 is a registered trademark of Molecular Probes, Inc. Alexa Fluor® 700 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
The UCHL1 antibody is commonly used in combination with antibodies against CD45RA to discern memory and naïve T cells. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections5 and formalin-fixed paraffin-embedded tissue sections4, Western blotting2, and immunoprecipitation3.
Application References:
1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. (FC) 2. Ishii T, et al. 2001. P. Natl. Acad. Sci. USA 98:12138. (WB) 3. Ponsford M, et al. 2001. Clin. & Exp. Immunol. 124:315. (IP) 4. Yamada M, et al. 1996. Stroke 27:1155. (IHC) 5. Sakkas LI, et al. 1998. Clin. and Diag. Lab. Immunol. 5:430. (IHC) 6. Baba N, et al. 2010. Int. Immunol. 22:237. PubMed 7. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed 8. Weiss L, et al. 2010. P. Natl. Acad. Sci. USA 107:10632. PubMed 9. Wu YY, et al. 2007. Infect. Immun. 75:4357. PubMed 10. Mozaffarian N, et al. 2008. Rheumatology 47:1335. PubMed 11. Roque S, et al. 2007. J. Immunol. 178:8028. PubMed 12. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Human peripheral blood lymphocytes stained with UCHL1 Alexa Fluor® 700
CD45RO is a 180 kD single chain membrane glycoprotein. It is a splice variant of tyrosine phosphatase CD45, lacking the A, B, and C determinants. The CD45RO isoform is expressed on activated and memory T cells, some B cell subsets, activated monocytes/macrophages, and granulocytes. CD45RO enhances both T cell receptor and B cell receptor signaling mediated activation. CD45 and its isoforms non-covalently associate with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1 and CD22. CD45 isoform expression can change in response to cytokines.
Structure:
Tyrosine phosphatases, type I transmembrane, 180 kD (isoform of CD45 containing none of the A, B, or C determinants)
Distribution:
Activated and memory T cells, B cell subsets, monocytes, macrophages, granulocytes
Function:
Enhances TCR and BCR signaling
Ligand Receptor:
CD22
Antigen References:
1. Thomas M. 1989. Annu. Rev. Immunol. 7:339. 2. Trowbridge I, et al. 1994. Annu. Rev. Immunol. 12:85.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-human CD45RO
Human peripheral blood lymphocytes stained with UCHL1 APC
FITC anti-human CD45RO
Human peripheral blood lymphocytes stained with UCHL1 FITC
PE anti-human CD45RO
Human peripheral blood lymphocytes stained with UCHL1 PE
Human peripheral blood lymphocytes stained with UCHL1 PE and CD45RA (HI100) Alexa Fluor® 647
PE/Cy5 anti-human CD45RO
Human peripheral blood lymphocytes stained with UCHL1 PE/Cy5
Purified anti-human CD45RO
Human peripheral blood lymphocytes stained with purified UCHL1, followed by anti-mouse IgGs FITC
Alexa Fluor® 488 anti-human CD45RO
Human peripheral blood lymphocytes stained with UCHL1 Alexa Fluor® 488
Alexa Fluor® 647 anti-human CD45RO
Human peripheral blood lymphocytes stained with UCHL1 Alexa Fluor® 647
Pacific Blue™ anti-human CD45RO
Human peripheral blood lymphocytes stained with UCHL1 Pacific Blue™
Alexa Fluor® 700 anti-human CD45RO
Human peripheral blood lymphocytes stained with UCHL1 Alexa Fluor® 700
Biotin anti-human CD45RO
Human peripheral blood lymphocytes stained with biotinylated UCHL1, followed by Sav-PE
Brilliant Violet 421™ anti-human CD45RO
Human peripheral blood lymphocytes were stained with CD45RA FITC and CD45RO (clone UCHL1) Brilliant Violet 421™ (top) or mouse IgG2a, κ Brilliant Violet 421™ isotype control (bottom).
PerCP/Cy5.5 anti-human CD45RO
Human peripheral blood lymphocytes stained with UCHL1 PerCP/Cy5.5
Brilliant Violet 570™ anti-human CD45RO
Human peripheral blood lymphocytes were stained with CD45RA FITC and CD45RO (clone UCHL1) Brilliant Violet 570™ (top) or mouse IgG2a, κ Brilliant Violet 570™ isotype control (bottom).
APC/Cy7 anti-human CD45RO
Human peripheral blood lymphocytes were stained with CD3 Pacific Blue™, CD45RA FITC and CD45RO (clone UCHL1) APC/Cy7 (top) or mouse IgG2a, κ APC/Cy7 isotype control (bottom). Data shown was gated on the CD3+ cell population.
PE/Cy7 anti-human CD45RO
Human peripheral blood lymphocytes were stained with CD45RA Brilliant Violet 421™ and CD45RO (clone UCHL1) PE/Cy7 (top) or mouse IgG2a, κ PE/Cy7 isotype control (bottom). Data shown was gated on the CD3+ population.
