The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
FC
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
LOU rat splenocytes stained with OX-52 Alexa Fluor® 647
Description:
Rat CD6 is a membrane molecule of 95-120 kd,expressedprimarily on all the T lymphocytes of alpha/beta or gamma/delta TCR.Rat NK cells express low levels of CD6.In immunoprecipitation studies,CD6 was found to co-precipitate with highly phorsphorylated CD5,and other protein kinases, such as Lck,Fyn ZAP70 and Itk.
Other Names:
OX-52 antigen, T12
Structure:
A membrane protein of about 100kd, 665 amino acids.
Distribution:
Positive on all peripheral T lymphocytes and thymocytes expressing either ab and gd T cell receptors. Rat NK cells express low level of CD6.
Interaction:
Rat CD6 associates with protein kinases of different families, including CD5, the Src kinases of Lck and Fyn, the ZAP-70 and Tec family kinase Itk.
Bioactivity/Activities:
Important role in the regulation of phosphorylation of CD5 and other molecules.
