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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for the relevant formats) include: immunohistochemical staining2,5,6 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded tissue sections, immunoprecipitation, intracellular flow cytometry3, immunofluorescence microscopy9, and Western blotting10.
Application References:
1. Ogata K, et al. 1985. J. Immunol. 135:2623. 2. Garcia R, et al. 1989. Am. J. Pathol. 134:733. 3. Landberg G, et al. 1990. Exp. Cell. Res. 187:111. 4. Waseem N, et al. 1990. J. Cell Sci. 96:121. 5. Yu C, et al. 1991. Histopathology 19:29. 6. Wilkins B, et al. 1992. J. Pathol. 166:45. 7. Yang W, et al. 1996. Human Pathol. 27:70. 8. Galkowska H, et al. 1996. Vet. Immunol. Immunopathol. 53:329. 9. Chou H-Y. E, et al. 2006. J. Biol. Chem. 10:1074. 10. Fulvio MD, et al. 2006. Oncogene 25:3932. 11. Eswarakumar VP and Schlessinger J. 2007. Proc. Natl. Acad. Sci. USA 104:3937.
Human peripheral blood lymphocytes fixed with 70% ethanol, then intracellular stained with PC10 Alexa fluor® 647
The PC10 monoclonal antibody reacts with proliferating cell nuclear antigen also known as PCNA or the DNA polymerase δ auxiliary protein. PCNA is a 36 kD trimeric ring that acts as a DNA-polymerase sliding clamp expressed in the nucleus of all proliferating cells. A prime function of PCNA appears to be increasing DNA polymerase δ processibility during elongation of the leading strand. PCNA is a useful marker for DNA synthesis and is highly conserved among most species, thus highlighting the very broad reactivity of this antibody.
Other Names:
Proliferating Cell Nuclear Antigen, DNA Polymerase δ Auxiliary Protein
RAD6-dependent DNA repair pathway; increases DNA polymerase d processibility during elongation of the leading strand
Ligand Receptor:
Ubiquitination, Sumoylation
Antigen References:
1. Travali S, et al. 1989. J. Biol. Chem. 264:7466. 2. Waseem N, et al. 1990. J. Cell Sci. 96:121. 3. Hall P, et al. 1990. J. Pathol. 162:285. 4. Landberg G, et al. 1991. Cancer Res. 51:4570. 5. Woods A, et al. 1991. Histopathol. 19:21. 6. Hoege C, et al. 2002. Nature 419:135. 7. Yue H, et al. 2003. World J. Gastroenterol. 9:377. 8. Shan B, et al. 2003. J. Biol. Chem. 278:44009.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
PE anti-PCNA
MOLT-4 cells fixed in 70% ethanol then stained with PC10 PE
Purified anti-PCNA
Hela cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-PCNA antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
Alexa Fluor® 488 anti-PCNA
MOLT-4 cells fixed in 70% ethanol then stained with PC10 Alexa Fluor® 488 or MOPC-173 Alexa Fluor® 488
Alexa Fluor® 647 anti-PCNA
Human peripheral blood lymphocytes fixed with 70% ethanol, then intracellular stained with PC10 Alexa fluor® 647
