Welcome to BioLegend’s one-of-a-kind advanced search. Search by any one of the single fields below or narrow down your searches by using multiple parameters. Looking for an anti-mouse NK cell antibody for IHC? Or looking for a FITC-conjugated antibody against a human marker for dendritic cells to use for flow cytometry? Only BioLegend’s advanced search can provide you with the best answers. If you have further questions, please contact our technical service team at 858-768-5801.
Use this document lookup tool to find Current Product Datasheets, Certificates of Analysis, or MSDS for any antibody products you have purchased from BioLegend.
Use the search box below to perform a site search of BioLegend.com powered by Google™.
The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® 647 is a registered trademark of Molecular Probes, Inc. Alexa Fluor® 647 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
The Notch receptors and their ligands are highly conserved from invertebrates to mammals.The Delta-like 1 (Dll1) is one of the four or five Notch ligands identified.The binding to Notch receptor results in the proteolysis of Notch and movement of intracellular portion of Notch into the nucleus.This translocation triggers a series of signaling process.Delta-like 1 is reported to be essential for the maintenance of marginal zone B cells in normal mice and engagement of Notch 1 by Dll 1 promotes differentiation of B lymphocytes to antibody-secreting cells.
Other Names:
Proto-oncogene of int-3
Structure:
Type 1 transmembrane receptor with a large extracellular domain consisting of 29 -36 EGF( epidermal growth factor) and three LNR (Lin12-Notch) repeats.
Distribution:
Notch 4 is preferentially expressed in endothelial cells.
Function:
Regulation of cell development, including myogenesis, neurogenesis, and lymphocyte development. Notch 4 seems to play a crucial role in vasculogenesis and angiogenesis.
Ligand Receptor:
Jagged 1, Jagged2, Delta-like 1, Delta-like3 and Delta-like 4
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse Notch 4
FN4/CHO cells (mouse Notch-4 transfected cells) stained with purified HMN4-14, followed by anti-Armenian hamster IgG PE
PE anti-mouse Notch 4
FN4/CHO (mouse Notch-4 transfected) cells stained with HMN4-14 PE
Alexa Fluor® 647 anti-mouse Notch 4
FN4/CHO (mouse Notch-4) transfected cell line stained with HMN4-14 Alexa Fluor® 647
APC anti-mouse Notch 4
FN4/CHO (mouse Notch-4) transfected cell line stained with HMN4-14 APC
Biotin anti-mouse Notch 4
Mouse Notch 4 transfected CHO cells were stained with biotinylated Notch 4 (clone HMN4-14) (filled histogram) or Armenian hamster IgG(open histogram), followed by Sav-APC.
