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The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. ** Alexa Fluor® 647 is a registered trademark of Molecular Probes, Inc. Alexa Fluor® 647 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for relevant formats) include: immunoprecipitation1 and blocking2, 3 of CHO cell or Con A-activated lymphoblast killing by NK cells.
Application References:
1. Mason LH, et al. 1998. J. Immunol. 160:4148. (IP) 2. Idris A, et al. 1999. Proc. Natl. Acad. Sci. 96:6330. (FC Block) 3. George TC, et al. 1999. J. Immunol. 162:2035. (FC Block) 4. Ortaldo JR, et al. 1999. J. Leukoc. Biol. 66:512. (FC) 5. Ortaldo JR, et al. 1998. J. Immunol. 160:1158. (FC)
C57BL/6 splenocytes stained with CD49b (DX5) PE and 4E5 Alexa Fluor® 647
C57BL/6 splenocytes stained with CD49b (DX5) PE and rat IgG2a Alexa Fluor® 647
Ly49D is a type II transmembrane protein belonging to the Ly49 family of NK-cell receptors in the C-type lectin superfamily. Ly49D is expressed on subsets of natural killer (NK) cells in C57BL/6, C3H/He and SJL mouse strains, but not Balb/c, DBA/2, AKR and CBA/J. The receptors form homodimers that bind to MHC class I alloantigens. Unlike inhibitory Ly49 family members, Ly49D lacks a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM) but contains a cytoplasmic immunoreceptor tyrosine-based activation motif. The activating receptor Ly49D delivers stimulatory signals for target cell lysis upon interacting with H2-Dd, Dr and Dsp2, but not with H2b or H2k class I antigens. Cross-linking of Ly49D activates intracellular kinase activity and calcium mobilization in NK cells. In 129/J mice, 4E5 antibody cross-reacts with Ly49O, LY49R and Ly49V.
Other Names:
Ly-49D
Structure:
A type II transmembrane protein belonging to the Ly49 family of NK-cell receptors in the C-type lectin superfamily.
Distribution:
Subsets of natural killer (NK) cells in C57BL/6, C3H/He and SJL, but not Balb/c, DBA/2, AKR and CBA/J.
Function:
Plays an important role in activating natural killer (NK) cell cytolytic activity.
Ligand Receptor:
MHC class I alloantigens
Antigen References:
1. Yokoyama WM and Seaman WE. 1993. Annu. Rev. Immunol. 11:613. 2. Takei F, et al. 1997. Immunol. Rev. 155:67. 3. Hanke T, et al. 1999. Immunity 11:67. 4. Mason LH, et al. 1996. J. Exp. Med. 184:2119.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse Ly49D
C57BL/6 splenocytes stained with CD49b (DX5) APC and purified 4E5 conjugated with FITC
C57BL/6 splenocytes stained with CD49b (DX5) APC and rat IgG2a FITC isotype control
FITC anti-mouse Ly49D
C57BL/6 splenocytes stained with CD49b (DX5) APC and 4E5 FITC
C57BL/6 splenocytes stained with CD49b (DX5) APC and rat IgG2a FITC isotype control
Alexa Fluor® 647 anti-mouse Ly49D
C57BL/6 splenocytes stained with CD49b (DX5) PE and 4E5 Alexa Fluor® 647
C57BL/6 splenocytes stained with CD49b (DX5) PE and rat IgG2a Alexa Fluor® 647
PE anti-mouse Ly49D
C57BL/6 splenocytes stained with NK1.1 (PK136) Alexa Fluor® 647 and 4E5 PE (top) or rat IgG2a PE isotype control (bottom)
