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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.06 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® 647 is a registered trademark of Molecular Probes, Inc. Alexa Fluor® 647 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
1. Jager A, et al. 2009. J. Immunol. 183:7169. PubMed 2. Angkasekwinai P, et. al. 2010. Nat. Immunol. 11:250. (ICFC) PubMed 3. Chang HC, et al. 2010. Nat. Immunol. 11:527. (ICFC) PubMed
CD4+ T cells from C57BL/6 mouse were cultured with anti-mouse CD3 and CD28 in the presence of recombinant IL-4 and TGF-beta for three days. Intracellular staining was performed following 5 hour restimulation with PdBU and ionomycin (in the presence of brefeldin A)
IL-9 is a potent, T cell-derived, T cell growth factor which can also enhance mast cell activity and IL-3- or IL-4- dependent proliferation of bone marrow-derived mast cells. IL-9 synergizes with erythropoietin to promote erythroid colony formation. IL-9 induces high affinity IgE receptor expression and granzyme A and B in murine T helper clones. The RM9A4 antibody reacts with mouse IL-9.
Upregulated by IL-1 after TCR activation, TNF-α downregulated by antibodies to IL-2
Cellular Sources:
IL-2 activated TH2 lymphocytes
Cellular Targets:
T lymphocytes, erythroid precursors
Receptors:
IL-9R
Bioactivity/Activities:
Potentiates production of IgG, IgM, and IgE by IL-4-induced B lymphocytes; regulates granzyme family proteases; enhances proliferation of bone marrow mast cells with IL-3; production of IL-6 by mast cells
Antigen References:
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Quesniaux V, et al. 1992. Research Immunology 143:385. 3. Renauld J, et al. 1993. Adv. Immunol. 54:79. 4. Yang Y, et al. 1992. Leuk. Lymphoma 8:441.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
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PE anti-mouse IL-9
FACS sorted naive CD4 T cells (CD44lo, CD25-) were cultured on plates coated with 1 µg/ml anti-CD3 and 10 µg/ml anti-CD28 in the presence of 10 ng/ml IL-4 and 5 ng/ml TGFβ for 3 days. Intracellular staining was performed following a 4h restimulation with PdBU/ionomycin/brefeldin. Data kindly provided by: Dr. Brigitta Stockinger, PhD
APC anti-mouse IL-9
CD4+ T cells from C57BL/6 mouse were cultured with anti-mouse CD3 and CD28 in the presence of recombinant IL-4 and TGF-beta for three days. Intracellular staining was performed following 5 hour restimulation with PdBU, Ionomycin, and brefeldin
Alexa Fluor® 647 anti-mouse IL-9
CD4+ T cells from C57BL/6 mouse were cultured with anti-mouse CD3 and CD28 in the presence of recombinant IL-4 and TGF-beta for three days. Intracellular staining was performed following 5 hour restimulation with PdBU and ionomycin (in the presence of brefeldin A)
