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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
ELISA Detection1-3 or ELISPOT Detection4-6: The biotinylated JES6-5H4 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified JES6-1A12 antibody (Cat. No. 503702/503704) as capture antibody and recombinant mouse IL-2 (Cat. No. 563001) as the standard. Flow Cytometry8-10: The fluorochrome-labeled JES6-5H4 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-2 -producing cells within mixed cell populations. View intracellular cytokine staining protocol Neutralization1,7: The LEAF™ purified antibody (Endotoxin in vivo and in vitro (Cat. No. 503812) is recommended for neutralization. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining2 of paraformaldehyde-fixed, saponin-treated frozen tissue sections, In Vivo Capture7, and immunocytochemistry. Note: For testing mouse IL-2 in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 431001 to 431006) are specially developed and recommended.
Application References:
1. Abrams J, et al. 1992. Immunol. Rev. 127:5. 2. Sander B, et al. 1993. J. Immunol. Meth. 166:201. 3. Abrams J. 1995. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.20. 4. Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.19. 5. Mo X, et al. 1995. J. Virol. 69:1288. 6. Karulin A, et al. 2000. J. Immunol. 164:1862. 7. Finkelman F, et al. 2003. Curr. Prot. Immunol. John Wiley & Sons New York. Unit 6.28. 8. Ko SY, et al. 2005. J. Immunol. 175:3309. PubMed 9. Kang SS and Allen PM. 2005. J. Immunol. 174:5382. 10. Lawson BR, et al. 2007. J. Immunol. 178:5366.
IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells. Additionally, IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes.
Other Names:
Interleukin-2, T cell growth factor (TCGF), Eosinophil differentiation factor (EDF), Killer cell helper factor (KHF), Macrophage-activating factor for cytotoxicity I (MAF-C I), Thymocyte differentiation factor (TDF)
Structure:
Cytokine; 15-30 kD (Mammalian)
Regulation:
Upregulated by NFAT; downregulated by TCF-8, CIF (colostrum inhibitory factor)
Cellular Sources:
T cells
Cellular Targets:
T cells, B cells, NK cells, LAK cells, monocytes, macrophages, oligodendrocytes
Receptors:
High affinity heterotrimer of IL-2Rα/β/γ, intermediate affinity homodimer IL-2Rα (CD25; p55; Tac) and heterodimer IL-2Rβ (CD122)/γ; γ-subunit (CD132) in common with IL-4R, IL-7R, IL-13R, IL-15R
Bioactivity/Activities:
Proliferation of T lymphocytes, B cells, anti-inflammatory, hematopoiesis, tumor surveillance
Antigen References:
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Taniguchi T, et al. 1993. Cell 73:5. 3. Nistico G. 1993. Prog. Neurobiol. 40:463. 4. Waldmann T, et al. 1993. Ann. NY Acad. Sci. 685:603.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-mouse IL-2
PMA+ionomycin stimulated C57BL/6 mouse splenocytes (6 hours) stained with anti-CD3 PE (17A2) and intracellularly stained with JES6-5H4 APC
Biotin anti-mouse IL-2
FITC anti-mouse IL-2
PMA-ionomycin-stimulated Balb/c mouse splenocytes intracellular stained with CD3 (17A2) PE and JES6-5H4 FITC
PE anti-mouse IL-2
PMA+ionomycin-stimulated C57BL/6 mouse splenocytes intracellular stained with with CD3 (17A2) APC and JES6-5H4 PE
Alexa Fluor® 488 anti-mouse IL-2
PMA+ionomycin-stimulated (6 hours) Balb/c mouse splenocytes intracellularly stained with CD3 (17A2) PE and JES6-5H4 Alexa Fluor® 488
PMA+ionomycin-stimulated C57BL/6 mouse splenocytes (6 hours) stained with B220 (RA3-6B2) PE and intracellularly stained with JES6-5H4 Alexa Fluor® 700
Pacific Blue™ anti-mouse IL-2
PMA+ionomycin-stimulated C57BL/6 mouse splenocytes (6 hours) intracellular stained with with CD3 (17A2) PE and JES6-5H4 Pacific Blue™
PerCP/Cy5.5 anti-mouse IL-2
PMA+ionomycin-stimulated (6 hours) C57BL/6 mouse splenocytes intracellular stained with B220 (RA3-6B2) APC and JES6-5H4 PerCP/Cy5.5
PE/Cy5 anti-mouse IL-2
PMA/Ionomycin-stimulated C57BL/6 mouse splenocytes (6 hours) intracellularly stained with JES6-5H4 PE/Cy5 and anti-mouse CD45R/B220 (RA3-6B2) APC
Brilliant Violet 421™ anti-mouse IL-2
C57BL/6 mouse splenocytes were stimulated with PMA + Ionomycin for 6 hours (in the presence of monensin), stained with CD3 FITC, fixed, permeabilized, and then stained with IL-2 (clone JES6-5H4) Brilliant Violet 421™ (top) or rat IgG2b, κ Brilliant Violet 421™ isotype control (bottom).
