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Alexa Fluor® 647 anti-mouse IgD Antibody
Alexa Fluor® 647 anti-mouse IgD Antibody
405707 25 µg $95.00     
405708 100 µg $225.00     
Product Details
Clone: 11-26c.2a
Isotype: Rat IgG2a, κ
Isotype Control:Alexa Fluor® 647 Rat IgG2a, κ Isotype Ctrl
Reactivity: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:

FC- Quality tested
IHC - Reported in the literature
Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
** Alexa Fluor® 647 is a registered trademark of Molecular Probes, Inc. Alexa Fluor® 647 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.



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Application
Notes:

The 11-26c.2a antibody reacts with immunoglobulin D in all tested mouse haplotypes. The antibody binds membrane IgD expressed on most B cells. The 11-26c.2a antibody neither induces proliferation of splenic B cells nor induces B cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining2,3 of acetone-fixed frozen sections.
Application
References:

1. Nitschke L, et al. 1993. P. Natl. Acad. Sci. 90:1887. (FC)
2. Weih D, et al. 2001. J. Immunol. 167:1909. (IHC)
3. Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC)
4. Ahuja A, et al. 2007. J. Immunol. 179:3351. (FC) PubMed
5. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
6. Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed
7. Tomyako MM, et al. 2010. J. Immunol. 185:7146. PubMed
8. Lahoud MH, et al. 2011. J. Immunol. 187:842. PubMed.
9. Gatto D, et al. 2011. J. Immunol. 187:4621. PubMed.
10. Vu T, et al. 2011. J. Immunol. 187:5032. PubMed.
11. Asselin-Labat ML, et al. 2011. 31:4609. PubMed.

C57BL/6 splenocytes stained with 11-26c.2a
C57BL/6 splenocytes stained with 11-26c.2a Alexa Fluor® 647


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Description:

Surface IgD is an important B cell differentiation marker.
Structure: Ig family
Distribution: B cells
Function: B cell differentiation
GeneID: 380797
Latest Publications: View the latest IgD articles on HighwirePress.com
UniProt: View information about IgD on UniProt.org
Keywords: Alexa Fluor® 647 anti-mouse IgD, 11-26c.2a, Alexa Fluor® 647, Mouse, Immunology, Antibodies
Related Products
DescriptionCloneApplications
Cell Staining BufferFC, ICC, ICFC
Alexa Fluor® 647 Rat IgG2a, κ Isotype CtrlRTK2758FC, ICFC
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
  • FITC anti-mouse IgD
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with 11-26c.2a FITC

  • PE anti-mouse IgD
    C57BL/6 splenocytes stained with 11-26c.2a PE

    C57BL/6 splenocytes stained with 11-26c.2a PE

  • Purified anti-mouse IgD
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with purified 11-26c.2a, followed by anti-rat IgG FITC

  • Alexa Fluor® 647 anti-mouse IgD
    C57BL/6 splenocytes stained with 11-26c.2a

    C57BL/6 splenocytes stained with 11-26c.2a Alexa Fluor® 647

  • PerCP/Cy5.5 anti-mouse IgD
    C57BL/6 splenocytes stained with 11-26c.2a

    C57BL/6 splenocytes stained with 11-26c.2a PerCP/Cy5.5

  • Pacific Blue™ anti-mouse IgD
    C57BL/6 splenocytes stained with 11-26c.2a

    C57BL/6 splenocytes stained with 11-26c.2a Pacific Blue™ and CD19 antibody (6D5) PE

    C57BL/6 splenocytes stained with rat

    C57BL/6 splenocytes stained with rat IgG2a Pacific Blue™ isotype and CD19 antibody (6D5) PE

  • APC anti-mouse IgD
    C57BL/6 splenocytes stained with 11-26c.2a

    C57BL/6 splenocytes stained with 11-26c.2a APC

  • APC/Cy7 anti-mouse IgD
    C57BL/6 splenocytes stained with 11-26c.2a

    C57BL/6 splenocytes stained with 11-26c.2a APC/Cy7

  • Alexa Fluor® 488 anti-mouse IgD
    C57BL/6 splenocytes were stained with

    C57BL/6 splenocytes were stained with B220 APC and IgD (11-26c.2a) Alexa Fluor® 488 (top) or rat IgG2a, κ Alexa Fluor® 488 isotype control (bottom).





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