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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
ELISA1-4,11,14 or ELISPOT5 Detection: The biotinylated XMG1.2 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified R4-6A2 antibody (Cat. No. 505702/505706) as the capture antibody and recombinant mouse IFN-γ (Cat. No. 575309) as the standard. ELISA or ELISPOT Capture: The purified XMG1.2 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated R4-6A2 antibody (Cat. No. 505704) as the detection antibody and recombinant mouse IFN-γ (Cat. No. 575309) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture. Flow Cytometry7,8,12,13,16: The fluorochrome-labeled XMG1.2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-γ-producing cells within mixed cell populations. View intracellular cytokine staining protocol Neutralization1-3,9,10: The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for neutralization of mouse IFN-γ bioactivity in vivo and in vitro (Cat. No. 505812). Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections6, and immunocytochemistry. Note: For testing mouse IFN-γ in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 430801 to 430806) are specially developed and recommended.
Application References:
1. Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA, Neut) 2. Sander B, et al. 1993. J. Immunol. Meth. 166:201. (ELISA, Neut) 3. Abrams J, et al. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20. (ELISA, Neut) 4. Yang X, et al. 1993. J. Immunoassay 14:129. (ELISA) 5. Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.19. (ELISPOT) 6. Sander B, et al. 1991. Immunol. Rev. 119:65. (IHC) 7. Ferrick D, et al. 1995. Nature 373:255. (FC) 8. Ko SY, et al. 2005. J. Immunol. 175:3309. (FC) PubMed 9. Peterson KE, et al. 2000. J. Virol. 74:5363. (Neut) 10. DeKrey GK, et al. 1998. Infect. Immun. 66:827. (Neut) 11. Dzhagalov I, et al. 2007. J. Immunol. 178:2113. (ELISA) 12. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC) 13. Lee JW, et al. 2006. Nature Immunol. 8:181. (FC) PubMed 14. Xu G, et al. 2007. J. Immunol. 179:5358. (ELISA) PubMed 15. Montfort M, et al.2004. J. Immunol. 173:4084. PubMed 16. Haring JS, et al. 2008. J. Immunol. 180:2855. (FC) PubMed 17. Jordan JM, et al. 2008. Infect Immun. 76:3717. PubMed 18. Tonkin DR, et al. 2008. J. Immunol. 181:4516. PubMed 19. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed 20. Cui Y, et al. 2009. Invest. Ophth. Vis. Sci. 50:5811. (FC) PubMed
Description:
Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells. The XMG1.2 antibody reacts with mouse interferon-γ (IFN-γ). The XMG1.2 antibody can neutralize the bioactivity of natural or recombinant IFN-γ.
Other Names:
Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma
Structure:
Cytokine; dimer; 40-80 kD (Mammalian)
Regulation:
Upregulated by IL-2, FGF-basic, EGF; downregulated by 1-α-25-Dihydroxy vitamin D3, dexamethasone
Cellular Sources:
CD8+ and CD4+ T cells, NK cells
Cellular Targets:
T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Receptors:
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Bioactivity/Activities:
Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APCs
Antigen References:
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321. 3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571. 4. Gray P, et al. 1987. Lymphokines 13:151.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
