The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
ICFC - Quality tested
Recommended Usage:
Each lot of this antibody is quality control tested by intracellular flow cytometry using our FOXP3 Fix/Perm protocol. For immunofluorescent staining, the suggested use of this reagent is 5 µl per 106 cells in 100 µl volume or 5 µl per 100 µl whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® 647 is a registered trademark of Molecular Probes, Inc. Alexa Fluor® 647 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
1. Thornton, A.M., et al., 2010. J. Immunol.. 184: 1. Pubmed
C57BL/6 splenocytes intracellular stained with 22F6 Alexa Fluor® 647 and CD4 (GK1.5) PE using FOXP3 Fix/Perm Buffer Set (cat# 421403).=
Human peripheral blood mononuclear cells (PBMCs) stained with human CD4 PE (clone RPA-T4) and 22F6 Alexa Fluor® 647
Description:
Helios is a member of the Ikaros family of zinc finger transcription factors. It contains a C-terminal region composed of 2 zinc-finger domains that mediate dimerization between the family members. Helios was originally cloned from a mouse thymoma line. It is mainly expressed in peripheral T cells and thymocytes. It is found at high levels in regulatory T cells from the Thymus but not peripherally induced Tregs. Helios plays an important role in T cell development and homeostasis. Overexpression of Helios profoundly alters αβ T cell differentiation and activation. It has been determined that silencing of Helios in B cells is critical for maintaining normal B cell function. Helios is involved in tumor immunity.
Other Names:
Structure:
A zinc finger nuclear transcription factor of the Ikaros family, containing 2 zinc-finger domains in C-terminal region.
Distribution:
Regulatory T cells (Tregs), conventional T cells, thymocytes.
Function:
Plays an important role in T cell development and homeostasis, involved in tumor immunity.
Antigen References:
1. Kelly, C.M., et al., 1998. Curr. Bio. 8:508. 2. Dovat, S., et al., 2005. J. Immunol. 175:3508. 3. Cortes, M., et al., 1999. Curr. Opin. Immunol. 11:167. 4. Cai, Q., et al., 2009. J. Immunol. 183:2303. 5. Zhang, Z., et al., 2007. Blood 109:2190. 6. Hahm, K., et al., 1998. Genes Dev. 12:782.
