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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. ** Alexa Fluor® 647 is a registered trademark of Molecular Probes, Inc. Alexa Fluor® 647 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunohistochemical staining of acetone-fixed frozen sections3. Clone AF6-88.5 is not suitable for immunohistochemical staining of formalin-fixed paraffin embedded sections.
The AF6-88.5 antibody reacts with H-2Kb MHC class I alloantigen expressed on nucleated cells from mice of the H-2Kb haplotype. H-2Kb is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The AF6-88.5 antibody does not cross-react with other haplotypes (d, f, j, k, p, q, r, s, u, v).
Other Names:
MHC class I
Structure:
MHC class I
Distribution:
Nucleated cells of the H-2Kb haplotype
Function:
Antigen presentation
Ligand Receptor:
CD3/TCR, CD8
Antigen References:
1. Watts C. 1997. Ann. Review Immunol. 15:821. 2. Pamer E, et al. 1998. Ann. Review Immunol. 16:323. 3. York I, et al. 1996. Ann. Rev. Immunol. 14:369.
Alexa Fluor® 647 anti-mouse H-2Kb, AF6-88.5, Alexa Fluor® 647, AF647, MHC class I, Mouse b haplotype, Does not cross-react with other haplotypes (e.g., d, f, j, k, p, q, r, s, u, v), Flow Cytometry, Immunofluorescence microscopy, Immunohistochemistry, Immunology, Antibodies
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-mouse H-2Kb
C57BL/6 mouse splenocytes stained with biotinylated AF6-88.5, followed by Sav-PE
FITC anti-mouse H-2Kb
C57BL/6 mouse splenocytes stained with AF6-88.5 FITC
PE anti-mouse H-2Kb
C57BL/6 mouse splenocytes stained with AF6-88.5 PE
Purified anti-mouse H-2Kb
C57BL/6 mouse splenocytes stained with purified AF6-88.5, followed by anti-mouse IgG2a FITC
Alexa Fluor® 488 anti-mouse H-2Kb
C57BL/6 mouse splenocytes stained with AF6-88.5 Alexa Fluor® 488
Alexa Fluor® 647 anti-mouse H-2Kb
C57BL/6 mouse splenocytes stained with AF6-88.5 Alexa Fluor® 647
Pacific Blue™ anti-mouse H-2Kb
C57BL/6 splenocytes stained with AF6-88.5 Pacific Blue™
PerCP/Cy5.5 anti-mouse H-2Kb
C57BL/6 splenocytes stained with AF6-88.5 PerCP/Cy5.5
APC anti-mouse H-2Kb
C57BL/6 mouse splenocytes stained with AF6-88.5 APC
