Welcome to BioLegend’s one-of-a-kind advanced search. Search by any one of the single fields below or narrow down your searches by using multiple parameters. Looking for an anti-mouse NK cell antibody for IHC? Or looking for a FITC-conjugated antibody against a human marker for dendritic cells to use for flow cytometry? Only BioLegend’s advanced search can provide you with the best answers. If you have further questions, please contact our technical service team at 858-768-5801.
Use this document lookup tool to find Current Product Datasheets, Certificates of Analysis, or MSDS for any antibody products you have purchased from BioLegend.
Use the search box below to perform a site search of BioLegend.com powered by Google™.
Alexa Fluor® 647 anti-mouse DC Marker (33D1) Antibody
Alexa Fluor® 647 anti-mouse DC Marker (33D1) Antibody
The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for other applications.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
33D1 antigen, also known as DCIR2 (dendritic cell inhibitory receptor 2), is reported to be a dendritic cell marker on subpopulation DC in spleen, thymus, Peyer’s patch. It is reported that most N418 (CD11c)+ cells in spleen are also 33D1 positive. Bone marrow dendritic cells upregulate 33D1 antigen expression after incubation with GM-CSF.
33D1 antigen was reported on dendritic cell populations from mouse thymus, spleen lymph nodes and Peyer patches. Bone marrow dendritic cells can be induced to express 33D1 antigen in the presence of GM-CSF. In mouse spleen, most N418+ cells are also 33D1 positive.
Function:
GM-CSF is reported to increase expression of 33D1 antigen on dendritic cells from bone marrow cells and IL-4 reported to down regulate the 33D1 antigen.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-mouse DC Marker (33D1)
BALB/c splenocytes stained with I-A/I-E (M5/114) FITC and biotinylated 33D1, followed by Sav-PE
PE anti-mouse DC Marker (33D1)
C57BL/6 splenocytes were stained with I-A/I-E APC and DC Marker (clone 33D1) PE (top) or rat IgG2b, κ PE isotype control (bottom).
FITC anti-mouse DC Marker (33D1)
C57BL/6 splenocytes were stained with I-A/I-E APC and DC Marker (clone 33D1) FITC (top) or rat IgG2b, κ FITC isotype control (bottom).
Alexa Fluor® 488 anti-mouse DC Marker (33D1)
C57BL/6 splenocytes stained with I-A/I-E (M5/114.15.2) APC and 33D1 Alexa Fluor® 488
C57BL/6 splenocytes stained with I-A/I-E (M5/115) APC and Alexa Fluor® 488 rat IgG2b isotype control
Alexa Fluor® 647 anti-mouse DC Marker (33D1)
C57BL/6 splenocytes stained with M5/114 (I-A/I-E) PE and 33D1 Alexa Fluor® 647
APC anti-mouse DC Marker (33D1)
C57BL/6 splenocytes were stained with I-A/I-E PE and DC Marker (clone 33D1) APC (top) or rat IgG2b, κ APC isotype control (bottom).
