The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
FC - Quality tested
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Application References:
Wang H et al. 2007. PNAS 104:13744
BALB/c mouse splenocytes stained with Miap301 Alexa Fluor® 647
Description:
CD47, also known as Integrin-Associated Protein (IAP) ,is a membrane protein of about 50 kd with an IgV-like extracelluluar domain, a five membrane-spanning segment and a short terminal cytoplasmic region.It is widely expressed on many cell types and often associated with beta 3 integrins.The significance of this molecules is recently drawing increasing attention.It has been reported that CD47 functions as a self marker.Red cells lacking CD47 were rapidly cleared from the bloodstream by splenic macrophages.By binding to SIRPalpha,CD47controls hemostatic innate immune functions,such as phagocytosis and cell trafficking
Other Names:
Integrin-associated protein, IAP, neurophilin,gp42, Rh-associated protein
Structure:
50kd plasma membrane protein of immunoglobulin supergene family, with an extracellular IgV-like domain of 120 amino acids, a five membrane spanning segment of 152 amino acids and a short cytoplasmic tail of 30 amino acids.
Distribution:
Broad tissue distribution, including haemopoietic cells, epithelial and endothelial cells, fibroblasts, erythrocytes and other tissue cells.
Function:
Adhesion molecules, immune regulation
Ligand Receptor:
SIRPalpha (CD172alpha, Signal regulatory protein alpha), thrombospondin
Interaction:
Associate with beta3 integrins
Bioactivity/Activities:
Upon interaction with SIRPalpha, controls innate immune effector function, such as host cell phagocytosis
Antigen References:
1. Brown E, et al. 1990. J Cell Biol 111:2785 2. Mawby WJ et al.<.i> 1994. Biochem J 304:525 3. Gao AG et al. 1996. J Biol Chem 271:21 4. Oldenborg PA et al. 2000. 288:2051 5. van Beck EM et al. 2005. J Immunol 175:7781
