The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for other applications.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for the relevant formats) include: immunoprecipitation1, complement-dependent cytotoxicity1,3, and immunohistochemistry2,5 of acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 103120).
Application References:
1. Ledbetter, J.A., et al., 1979. Immunol. Rev. 47:63. 2. Simon, D.I., et al., 2000. J. Clin. Invest. 105:293. 3. Seaman, W.E., 1983. J. Immunol. 130:1713. 4. Podd, B.S., et al., 2006. J. Immunol. 176: 6532. 5. Cornet, A., et al., 2001. Proc Natl Acad Sci U S A 98(23):13306. 6. Haynes, N.M., et al., 2007. J. Immunol. 179:5099.
C57BL/6 mouse splenocytes stained with 30-F11 Alexa Fluor® 647
Description:
CD45 is a 180-240 kD glycoprotein, also known as the leukocyte common antigen (LCA), T200 or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell, as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
Other Names:
T200, Ly-5, LCA
Structure:
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution:
All hematopoietic cells except mature erythrocytes and platelets
Function:
Phosphatase, T and B cell activation
Ligand Receptor:
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Antigen References:
1. Barclay, A., et al., 1997. The Leukocyte Antigen FactsBook, Academic Press. 2. Trowbridge, I.S., et al., 1993. Annu. Rev. Immunol. 12:85. 3. Kishihara, K., et al., 1993. Cell 74:143. 4. Pulido, R., et al., 1988. J. Immunol. 140:3851.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering/). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
