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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. ** Alexa Fluor® 647 is a registered trademark of Molecular Probes, Inc. Alexa Fluor® 647 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
The HM34 antibody does not stain bone marrow cells like some other mouse CD34 antibodies, probably because the antibody recognizes a different epitope from other mAbs.
NIH/3T3 cell line stained with HM34 Alexa Fluor® 647
CD34 is a highly glycosylated hematopoietic progenitor antigen.Two isoforms of CD34 have been reported. CD34 is expressed on hematopoietic progenitors, as well as endothelial cells, brain and testis. CD34 is thought to function as an adhesion molecule for attachment of stem cells to extracellular matrix or stromal cells.
Other Names:
Mucosialin
Structure:
Type I membrane protein, highly glycosylated, 75-120 kd, two isoforms reported.
Possible adhesion molecules thought to function in early hematopoiesis by mediating attachment of stem cells to bone marrow extracellular matrix or stromal cells. Present carbohydrate ligands to selectins.
Ligand Receptor:
L-selectin, and others.
Antigen References:
1. Garlanda C, et al. 1997. Eur J Cell Biol 73:368 2. Brown J, et al. 1991. Int Immunol 3:175
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse CD34
NIH/3T3 cell line stained with purified HM34, followed by biotinylated anti-Armenian hamster IgG and Sav-PE
Biotin anti-mouse CD34
NIH/3T3 cell line stained with HM34 biotin, followed by Sav-PE
Alexa Fluor® 647 anti-mouse CD34
NIH/3T3 cell line stained with HM34 Alexa Fluor® 647
PerCP/Cy5.5 anti-mouse CD34
Bend.3 mouse endothelial cells stained with HM34 PerCP/Cy5.5
PE anti-mouse CD34
Fibroblast NIH/3T3 cells stained with HM34 PE
APC anti-mouse CD34
Mouse fibroblast NIH/3T3 cells stained with HM34 APC.
