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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647 and unconjugated antibody.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. ** Alexa Fluor® 647 is a registered trademark of Molecular Probes, Inc. Alexa Fluor® 647 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
The 89B3A5 antibody recognizes mouse CD309 also known as vascular endothelial growth factor receptor 2, VEGFR2, KDR, protein tyrosine kinase receptor flk-1, and fetal liver kinase-1. Flk-1 is a member of the tyrosine protein kinase family, sub-family CSF-1/PDGF, that contains a single pass transmembrane receptor with a protein kinase domain and seven immunoglobulin-like domains in the extracellular region. Flk-1 is expressed at high levels in adult heart, lung, kidney, brain, and skeletal muscle; other tissues express at lower levels. Flk-1 is a receptor for VEGF or VEGFC; ligand binding plays a key role in vascular development and vascular permeability. The 89B3A5 antibody has been shown to be useful for flow cytometry.
Member of the tyrosine protein kinase family, sub-family CSF-1/PDGF. Single pass transmembrane receptor containing a protein kinase domain and seven immunoglobulin-like domains.
Distribution:
Expressed at high levels in adult heart, lung, kidney, brain, and skeletal muscle; other tissues express at lower levels.
Function:
Receptor for VEGF or VEGFC. Ligand binding plays a key role in vascular development and vascular permeability.
Ligand Receptor:
VEGF, VEGFC
Antigen References:
1. Patterson C, et al. 1995. J. Biol. Chem. 270:23111. 2. Quinn TP, et al. 1993. Proc. Natl. Acad. Sci. USA 90:7533.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse CD309 (VEGFR2, Flk-1)
Mouse FLK-1 transfected cells stained with purified 89B3A5, followed by biotinylated anti-rat IgG and Sav-PE
Biotin anti-mouse CD309 (VEGFR2, Flk-1)
Mouse FLK-1 transfected cells stained with biotinylated 89B3A5, followed by Sav-PE
PE anti-mouse CD309 (VEGFR2, Flk-1)
Mouse FLK-1 transfected cells stained with 89B3A5 PE
Alexa Fluor® 488 anti-mouse CD309 (VEGFR2, Flk-1)
Mouse FLK-1 transfected cells stained with 89B3A5 Alexa Fluor® 488
Alexa Fluor® 647 anti-mouse CD309 (VEGFR2, Flk-1)
Mouse FLK-1 transfected cells stained with 89B3A5 Alexa Fluor® 647
Pacific Blue™ anti-mouse CD309 (VEGFR2, Flk-1)
Mouse FLK-1 transfected cells stained with 89B3A5 Pacific Blue™
PerCP anti-mouse CD309 (VEGFR2, Flk-1)
FLK-1 transfected cells stained with 89B3A5 PerCP
PerCP/Cy5.5 anti-mouse CD309 (VEGFR2, Flk-1)
FLK-1 transfected cells stained with 89B3A5 PerCP/Cy5.5
