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The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647 and unconjugated antibody.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. ** Alexa Fluor® 647 is a registered trademark of Molecular Probes, Inc. Alexa Fluor® 647 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for relevant formats) include: Immunoprecipitation1,2 and blocking3,5 of OT-I T cell responses in vitro and incidence of cerebral malaria in vivo caused by P. berghei ANKA (PbA) infection.
Application References:
1. Hurchla MA, et al. 2005. J. Immunol. 174:337. (IP FC) 2. Sedy JR, et al. 2005. Nat. Immunol. 6:90. (IP) 3. Lepenies B, et al. 2007. J. Immunol. 179:4093. (Block) 4. Tao R, et al. 2005. J. Immunol. 175:5774. (FC) 5. Albring J, et al. 2010. J. Exp. Med. 207:2551. (Block)
C57BL/6 splenocytes stained with CD45R/B220 (RA3-6B2) PE and 6A6 Alexa Fluor® 647
B and T lymphocyte attenuator (BTLA) is an Ig superfamily coinhitory receptor with structural similarity to programmed cell death 1 (PD-1) and CTLA-4. BTLA is expressed on B cells, T cells, macrophages, dendritic cells, NKT cells, and NK cells. Engagement of BTLA by its ligand Herpes Virus Entry Mediator (HVEM) is critical for negatively regulating immune response. The absence of BTLA with HVEM inhibitory interactions leads to increased experimental autoimmune encephalomyelitis severity, enhanced rejection of partially mismatched allografts, an increased CD8+ memory T cell population, increased severity of colitis, reduced effectiveness of T regulatory cells. BTLA takes an important role in the induction of peripheral tolerance of both CD4+ and CD8+ T cells in vivo. Tolerant T cells have significant up-regulated expression of BTLA compared with effectors and naïve T cells. BTLA may cooperate with CTLA-4 and PD-1 to control T cell tolerance and autoimmunity. It was reported that BTLA may regulate T cell function by binding to B7-H4. But further studies are needed to confirm. The existence of three distinct BTLA alleles was reported. The BTLA antibody reacts only with the C57BL/6 allele of BTLA but not the BALB/c allele of BTLA.
Other Names:
BTLA, B and T lymphocyte attenuator
Structure:
An Ig superfamily coinhitory receptor with structural similarity to programmed cell death 1 (PD-1) and CTLA-4.
Distribution:
BTLA is expressed on a wide number of lymphocytes in mice. It is most highly expressed on B cells, followed by CD4+T cells, lower express on CD8+ T cells, macrophages, dendritic cells, NKT cells, and NK cells.
Function:
BTLA functions as a negative regulator of T cell activation and proliferation, attenuate B cell proliferation upon associating with its known ligand, Herpes Virus Entry Mediator (HVEM).
Ligand Receptor:
Herpes Virus Entry Mediator (HVEM)
Antigen References:
1. Liu X, et al. 2009. J. Immunol. 182:4516. 2. Miller ML, et al. 2009. J. Immunol. 183:32. 3. Sun Y, et al. 2009. J. Immunol. 183:1946. 4. Vendel AC, et al. 2009. J. Immunol. 182:1509. 5. Watanabe N, et al. 2003. Nat. Immunol. 4:670.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
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Purified anti-mouse CD272 (BTLA)
C57BL/6 splenocytes stained with CD45R/B220 (RA3-6B2) PE and CD272 (clone 6A6) Alexa Fluor® 647.
LEAF™ Purified anti-mouse CD272 (BTLA)
C57BL/6 splenocytes stained with CD45R/B220 (RA3-6B2) PE and purified 6A6 conjugated with Alexa Fluor® 647
Alexa Fluor® 647 anti-mouse CD272 (BTLA)
C57BL/6 splenocytes stained with CD45R/B220 (RA3-6B2) PE and 6A6 Alexa Fluor® 647
