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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. ** Alexa Fluor® 647 is a registered trademark of Molecular Probes, Inc. Alexa Fluor® 647 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
1. Ng LG, et al. 2006. Eur. J. Immunol. 36:1837. 2. Ng LG, et al. 2004. J Immunol. 173:807. 3. Batten M, et al. 2000. J. Exp. Med. 192:1453. 4. Fu L, et al. 2009. Blood 113:4627.
C57BL/6 splenocytes double stained with 7H22-E16 Alexa Fluor® 647 (upper panel) or rat IgG1 isotype control Alexa Fluor 647® (lower panel) and B220 (RA3-6B2) PE
BAFF (B cell-activating factor belonging to the TNF family) receptor (BAFF-R) is one of the three receptors that bind to BAFF. It is a type III transmembrane TNF receptor family member. BAFF-R is expressed at high levels in resting B cells and at lower levels in activated B cells, a small subset of activated/memory CD4+ T cells. It is a major mediator of BAFF dependent co-stimulatory response in peripheral B and T cells. It was reported that the mice expressing defective BAFF-R have disrupted B cell maturation. BAFF receptor appears to be particularly important for the regulation of normal B cell survival and maturation, as well as in the pathophysiology of aggressive B-cell malignancies and autoimmune diseases.
A type III transmembrane TNF receptor family member
Distribution:
Expressed on most B cells, a small subset of CD4+ T cells.
Function:
Play an importance role in B cell development, maturation, and survival.
Ligand Receptor:
BAFF (BLyS)
Antigen References:
1. Thompson JS, et al. 2001. Science 293:2108. 2. Schiemann B, et al. 2001. Science 293:2111. 3. Batten M, et al. 2000. J. Exp. Med. 192:1453. 4. Fu L, et al. 2009. Blood 113:4627.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
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Purified anti-mouse CD268 (BAFF-R)
C57BL/6 mouse splenocytes stained with purified 7H22-E16 conjugated to PE (upper) or rat IgG1 PE isotype control (lower panel) and CD45R/B220 APC
PE anti-mouse CD268 (BAFF-R)
C57BL/6 mouse splenocytes stained with 7H22-E16 PE (upper) or rat IgG1 PE isotype control (lower panel) and CD45R/B220 APC
Alexa Fluor® 647 anti-mouse CD268 (BAFF-R)
C57BL/6 splenocytes double stained with 7H22-E16 Alexa Fluor® 647 (upper panel) or rat IgG1 isotype control Alexa Fluor 647® (lower panel) and B220 (RA3-6B2) PE
