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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® 647 is a registered trademark of Molecular Probes, Inc. Alexa Fluor® 647 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
The RM134L antibody can block the costimulatory activity of OX40L. Additional reported applications (for the relevant formats) include: immunohistochemical staining3 of acetone-fixed frozen sections, and in vivo and in vitro blocking1,2,4 of OX-40L-OX-40 functional interaction. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 108808).
Application References:
1. Akiba H, et al. 1999. J. Immunol. 162:7058. (Block) 2. Akiba H, et al. 2000. J. Exp. Med. 191:375. (Block) 3. Hoshino A, et al. 2003. Eur. J. Immunol. 33:861. (IHC) 4. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Block) 5. Zheng X, et al. 2010. Invest Ophthalmol Vis Sci. 51:3076. PubMed
C57BL/6 splenocytes were stimulated with anti-mouse IgM and CD40 antibodies for 3 days, then stained with OX40L (clone RM134L) Alexa Fluor® 647 (filled histogram) or rat IgG2b Alexa Fluor® 647 isotype control (open histogram).
CD252 is a 35 kD member of the TNF superfamily and is also known as OX40L and CD134L. The OX40 ligand is expressed on activated B cells and antigen presenting cells. OX40 ligand interacts with OX40 antigen (CD134) expressed predominantly on activated T cells to increase proliferation and IL-2 production, and to enhance proliferation and immunoglobulin secretion in activated B cells.
Other Names:
OX-40L, CD134L, CD252, TNFSF4
Structure:
NGF/TNF superfamily, 35 kD
Distribution:
Activated B cells, antigen presenting cells
Function:
B-cell/T-cell interaction, costimulation
Ligand Receptor:
OX40(CD134)
Antigen References:
1. Akiba H, et al. 1999. J. Immunol. 162:7058. 2. Stüber E, et al. 1995. Immunity 2:507. 3. Baum PR, et al. 1994. EMBO J. 13:3992.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-mouse CD252 (OX40L)
C57BL/6 splenocytes were stimulated with anti-mouse IgM+CD40 antibodies for 3 days, then stained with biotinylated CD252 (clone RM134L) (filled histogram) or rat IgG2b, κ isotype control (open histogram), followed by Sav-PE.
LEAF™ Purified anti-mouse CD252 (OX40L)
C57BL/6 splenocytes were stimulated with anti-mouse IgM and CD40 antibodies for 3 days, then stained with LEAF™ purified OX40L (clone RM134L) (filled histogram) or rat IgG2b isotype control (open histogram), followed by biotinylated anti-rat IgG and Sav-PE.
PE anti-mouse CD252 (OX40L)
C57BL/6 splenocytes were stimulated with anti-mouse IgM and CD40 antibodies for 3 days, then stained with OX40L (clone RM134L) PE (filled histogram) or rat IgG2b PE isotype control (open histogram).
Purified anti-mouse CD252 (OX40L)
C57BL/6 splenocytes were stimulated with anti-mouse IgM and CD40 antibodies for 3 days, then stained with purified OX40L (clone RM134L) (filled histogram) or rat IgG2b isotype control (open histogram), followed by biotinylated anti-rat IgG and Sav-PE.
Alexa Fluor® 647 anti-mouse CD252 (OX40L)
C57BL/6 splenocytes were stimulated with anti-mouse IgM and CD40 antibodies for 3 days, then stained with OX40L (clone RM134L) Alexa Fluor® 647 (filled histogram) or rat IgG2b Alexa Fluor® 647 isotype control (open histogram).
