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The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. ** Alexa Fluor® 647 is a registered trademark of Molecular Probes, Inc. Alexa Fluor® 647 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1, Western Blot1-3, and immunoprecipitation of bone marrow dendritic cell extracts2.
Application References:
1. Witmer-Pack MD, et al. 1995. Cell. Immun. 163:157. (IHC, WB) 2. Swiggard WJ, et al. 1995. Cell. Immun. 165:302. (WB, IP) 3. Bonifaz LC, et al. 2004. J. Exp. Med. 199:815. (WB) 4. Yamazaki S, et al. 2002. J. Immunol. 181:6923. (FC) 5. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC)
Bone marrow derived-dendritic cells from C57BL/6 mouse stained with NLDC-145 Alexa Fluor® 647
C57BL/6 mouse bone marrow cells stained with Gr-1 (RB6-8C5) FITC and NLDC-145 Alexa Fluor® 647
C57BL/6 mouse bone marrow cells stained with Gr-1 (RB6-8C5) FITC and rat IgG2a Alexa Fluor® 647 isotype control
CD205, also known as DEC-205, is a 205 kD integral membrane protein homologous to the macrophage mannose receptor. It is a type I cell surface protein that belongs to the C-type lectin family. CD205 is expressed at high levels by dendritic cells and thymic epithelial cells. It is also expressed by a number of other cell types, such as B lymphocytes, macrophages, Langerhans cells, bone marrow stromal cells, granulocytes, epithelial cells of pulmonary airways, and the capillaries of the brain. CD205 is a novel endocytic receptor used by dendritic cells and thymic epithelial cells to direct captured antigens from the extracellular space to specialized antigen processing. It mediates antigen uptake and presentation and cross-presentation to T cells. It has been reported that CD205 acts as a recognition receptor for dying cells, potentially provides an important pathway for the uptake of self-antigen in the intrathymic environment, and is involved in peripheral tolerance. Antibody-mediated antigen-targeting via the DEC-205 receptor increases the efficiency of vaccination for T cell immunity.
Other Names:
DEC-205
Structure:
A 205 kD type I tramsmembrane protein belonging to the C-type lectin family and homologous to the macrophage mannose receptor.
Distribution:
Expressed at high levels by dendritic cells and thymic epithelial cells. Also expressed by B lymphocytes, macrophages, Langerhans cells, bone marrow cells and epithelial cells.
Function:
Mediates endocytosis, antigen uptake, presentation, and cross-presentation to T cells.
Antigen References:
1. Jiang WP, et al. 1995. Nature 375:151. 2. Small M and Kraal G. 2003. Int. Immunol. 15:197. 3. Shrimpton RE, et al. 2009. Mol. Immunol. 46:1229.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse CD205 (DEC-205)
Bone marrow derived-dendritic cells from C57BL/6 mouse stained with purified NLDC-145 conjugated with Alexa Fluor® 647
Alexa Fluor® 647 anti-mouse CD205 (DEC-205)
Bone marrow derived-dendritic cells from C57BL/6 mouse stained with NLDC-145 Alexa Fluor® 647
C57BL/6 mouse bone marrow cells stained with Gr-1 (RB6-8C5) FITC and NLDC-145 Alexa Fluor® 647
C57BL/6 mouse bone marrow cells stained with Gr-1 (RB6-8C5) FITC and rat IgG2a Alexa Fluor® 647 isotype control
APC anti-mouse CD205 (DEC-205)
C57BL/6 bone marrow cells stained with Gr-1 (RB6-8C5) FITC and NLDC-145 APC
C57BL/6 bone marrow cells stained with Gr-1(RB6-8C5) FITC and rat IgG2a APC isotype control
PerCP/Cy5.5 anti-mouse CD205 (DEC-205)
C57BL/6 mouse bone marrow cells stained with NLDC-145 PerCP/Cy5.5 and Gr-1 (RB6-8C5) APC
PE/Cy7 anti-mouse CD205 (DEC-205)
C57BL/6 mouse bone marrow cells stained with NLDC-145 PE/Cy7 and Gr-1 (RB6-8C5) APC
PE anti-mouse CD205 (DEC-205)
C57BL/6 mouse bone marrow cells were stained with Ly-6G/Ly-6C APC and CD205 (clone NLDC-145) PE (top) or rat IgG2a PE isotype control (bottom). Data shown was gated on the myeloid population.
Biotin anti-mouse CD205 (DEC-205)
C57BL/6 mouse bone marrow cells were stained with Ly-6G/Ly-6C APC and biotinylated CD205 (clone NLDC-145, top) or rat IgG2a isotype control (bottom), followed by Sav-PE. Data shown was gated on the myeloid population.
