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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. ** Alexa Fluor® 647 is a registered trademark of Molecular Probes, Inc. Alexa Fluor® 647 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
CXCR2 is a 7-transmembrane G protein–coupled receptor that is activated by CXC chemokines containing the ELR (Glu-Leu-Arg) motif, including murine CXCL1 (keratinocyte-derived chemokine; KC) CXCL2 (macrophage inflammatory protein 2; MIP-2), CXCL3 (DC inflammatory protein-1; Dcip1) and CXCL5 (LIX). CXCR2 is expressed on neutrophils, keratinocytes, mast cells, eosinophils, macrophages, and lung endothelial cells (1-3). Homing of mast cell progenitors (MCps) to the mouse small intestine involves CXCR2, vascular cell adhesion molecule-1 (VCAM-1), and the interaction of α4β7 integrin with mucosal addressin cellular adhesion molecule-1 (MAdCAM-1) (4). In addition, CXCR2 regulates endothelial VCAM-1 expression, MCp migration and the levels of intraepithelial MC in the lung of aerosolized, antigen-challenged mice (5).
neutrophils, keratinocytes, mast cells, eosinophils, macrophages, endothelial and epithelial cells
Function:
Chemotaxis, adhesion molecule-up regulation, angiogenesis. CXCR2 expression on neutrophils plays a crucial role in their migration to sites of inflammation
Ligand Receptor:
CXCL1, CXCL2, CXCL3, and CXCL5
Antigen References:
1) Nolan KF, et al.JI 172:2201-2209 2004. 2) Reutershan J et al, J Clin Invest 116:695-702 2006 3) Nicholls et al, Mol Pharmacol August 19 2008 4) Abonia JP et al, Blood 105:4308-4313 2005 5) Hallgren J et al, P. Natl. Acad. Sci. USA 104:20478-20483 2007.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Alexa Fluor® 647 anti-mouse CD182 (CXCR2)
C57BL/6 bone marrow cells stained with RB6-8C5 (Gr-1) FITC and TG11/CXCR2 Alexa Fluor® 647
PerCP/Cy5.5 anti-mouse CD182 (CXCR2)
C57BL/6 bone marrow cells stained with RB6-8C5 (Gr-1) FITC and TG11/CXCR2 PerCP/Cy5.5
