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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. ** Alexa Fluor® 647 is a registered trademark of Molecular Probes, Inc. Alexa Fluor® 647 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for the relevant formats) include: immunoprecipitation1, and blocking Fc-mediated reactions in functional studies2,4,5. For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with Purified anti-CD16/CD32 antibody (≤ 1.0 µg per 106 cells in 100 µl volume) for 5-10 minutes on ice prior to immunostaining. The LEAF™Purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 101310).
Application References:
1. Personal communication (IP) 2. Oliver AM, et al. 1999. Hybridoma 18:113. (Block) 3. Brummel R and Lenert P. 2005. J. Immunol. 174:2429. 4. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Block) 5. Clements JL, et al. 2006. J. Immunol. 177:905. (Block) 6. Mohamed W, et al. 2010. Infect Immun. 78:3306. PubMed
C57BL/6 mouse splenocytes stained with Alexa Fluor® 647
CD16 is the low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses. The 93 mAb is specific to the common epitope of CD16/CD32. It is useful for blocking non-specific binding of immunoglobulin to the Fc receptors.
Other Names:
Fcγ R III/II, Ly-17
Structure:
Ig superfamily, 40-60 kD
Distribution:
B cells, monocyte/macrophages, NK cells, neutrophils, mast cells, dendritic cells
Function:
Low affinity receptors for IgG
Ligand Receptor:
IgG
Antigen References:
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Unkeless JC. 1989. J. Clin. Invest. 83:355. 3. Qiu WQ, et al. 1990. Science 248:732.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-mouse CD16/32
C57BL/6 mouse splenocytes stained with biotinylated 93, followed by Sav-PE
FITC anti-mouse CD16/32
C57BL/6 mouse splenocytes stained with 93 FITC
LEAF™ Purified anti-mouse CD16/32
C57BL/6 mouse splenocytes stained with LEAF™ purified 93, followed by anti-rat IgGs FITC
PE anti-mouse CD16/32
C57BL/6 mouse splenocytes stained with 93 PE
Purified anti-mouse CD16/32
C57BL/6 mouse splenocytes stained with purified 93, followed by anti-rat IgGs FITC
Alexa Fluor® 647 anti-mouse CD16/32
C57BL/6 mouse splenocytes stained with Alexa Fluor® 647
PE/Cy7 anti-mouse CD16/32
C57BL/6 splenocytes stained with 93 PE/Cy7
PerCP/Cy5.5 anti-mouse CD16/32
C57BL/6 splenocytes stained with 93 PerCP/Cy5.5
APC anti-mouse CD16/32
C57BL/6 splenocytes stained with 93 APC
APC/Cy7 anti-mouse CD16/32
Balb/c splenocytes stained with 93 APC/Cy7 and CD3σ (145-2C11) PE
Balb/c splenocytes stained with rat IgG2a (RTK2758) APC/Cy7 isotype control and CD3σ (145-2C11) PE
